中性蛋白酶、碱性蛋白酶和木瓜蛋白酶水解乳清蛋白浓缩物对 Caco-2 细胞铁吸收的影响。
Effect of neutrase, alcalase, and papain hydrolysis of whey protein concentrates on iron uptake by Caco-2 cells.
机构信息
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.
出版信息
J Agric Food Chem. 2010 Apr 28;58(8):4894-900. doi: 10.1021/jf100055y.
Effects of enzymatic hydrolysates of whey protein concentrates (WPC) on iron absorption were studied using in vitro digestion combined with Caco-2 cell models for improved iron absorption. Neutrase- and papain-treated WPC could improve iron absorption; especially hydrolysates by Neutrase could significantly increase iron absorption to 12.8% compared to 3.8% in the control. Hydrolysates by alcalase had negative effects to the lowest at 0.57%. Two new bands at molecular weights (MW) around and below 10 kDa occurred at tricine-SDS-PAGE of hydrolysates by Neutrase, and one new band at MW below 10 kDa occurred in hydrolysates by papain. No new band was observed in hydrolysates by alcalase. Concentration of free amino acids indicated that, except for tyrosine and phenylalanine, amino acids in papain-treated hydrolysates were higher than that of alcalase, and no cysteine and proline were found in hydrolysates by alcalase. The results suggested that hydrolysate by Neutrase-treated WPC is a promising facilitator for iron absorption. Peptides of MW around and lower than 10 kDa and aspartic acid, serine, glutamic acid, glycin, cysteine, histidine, and proline may be contributors to enhancement.
研究了乳清蛋白浓缩物（WPC）的酶解产物对铁吸收的影响，使用体外消化结合 Caco-2 细胞模型来提高铁吸收。中性蛋白酶和木瓜蛋白酶处理的 WPC 可以提高铁吸收；特别是中性蛋白酶水解产物可使铁吸收显著增加到 12.8%，而对照组仅为 3.8%。碱性蛋白酶水解产物的效果最差，为 0.57%。中性蛋白酶水解产物的 tricine-SDS-PAGE 中出现了两个新的分子量（MW）约为 10 kDa 以下的条带，而木瓜蛋白酶水解产物中出现了一个新的 MW 低于 10 kDa 的条带。碱性蛋白酶水解产物中没有观察到新的条带。游离氨基酸浓度表明，除了酪氨酸和苯丙氨酸外，木瓜蛋白酶处理的水解产物中的氨基酸含量高于碱性蛋白酶，而碱性蛋白酶水解产物中没有半胱氨酸和脯氨酸。结果表明，WPC 的 Neutrase 处理水解产物是一种很有前途的铁吸收促进剂。MW 约为 10 kDa 以下的肽以及天冬氨酸、丝氨酸、谷氨酸、甘氨酸、半胱氨酸、组氨酸和脯氨酸可能是增强铁吸收的原因。
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