商业蛋白酶制备猪副产品胶原蛋白水解物的抗氧化和抗衰老活性：水解和超滤的影响。
Anti-Oxidative and Anti-Aging Activities of Porcine By-Product Collagen Hydrolysates Produced by Commercial Proteases: Effect of Hydrolysis and Ultrafiltration.
机构信息
Department of Food Science and Biotechnology, Sejong University, 209 Neungdong-ro, Seoul 05006, Korea.
Animal Resources Research Center, Konkuk University, 120 Neungdong-ro, Seoul 05029, Korea.
出版信息
Molecules. 2019 Mar 20;24(6):1104. doi: 10.3390/molecules24061104.
To investigate methods for improving the processing of porcine waste, porcine skin was hydrolyzed using different commercially available proteases (Alcalase, Flavorzyme, Neutrase, Bromeline, Protamex, and Papain) under several optimal conditions. Following enzymatic hydrolysis, the collagen hydrolysates (CHs) were fractionated by molecular weight (3 kDa) via membrane ultrafiltration. The CHs were analyzed for physical properties (pH, protein recovery, free amino group content, molecular weight distribution, and amino composition) as well as for functional properties (antioxidant activities and anti-aging activities). Among the CHs, CHs hydrolyzed by Alcalase (CH-Alcalase) exhibited the highest degree of hydrolysis compared to other CHs. Both "CH-Alcalase" and "CH-Alcalase < 3 kDa" fractions showed a considerably high antioxidant activity and collagenase inhibition activity. Therefore, resulting bioactives have potential for development as antioxidants and anti-aging ingredients in the food, cosmetics, and pharmaceuticals, from animal by-products.
为了研究提高猪废物处理方法，使用不同的市售蛋白酶（碱性蛋白酶、风味蛋白酶、中性蛋白酶、菠萝蛋白酶、复合蛋白酶和木瓜蛋白酶）在几种最佳条件下对猪皮进行水解。酶解后，通过膜超滤按分子量（3 kDa）对胶原蛋白水解物（CH）进行分级。对 CH 的物理性质（pH 值、蛋白质回收率、游离氨基含量、分子量分布和氨基酸组成）和功能性质（抗氧化活性和抗衰老活性）进行分析。在 CH 中，与其他 CH 相比，碱性蛋白酶水解的 CH（CH-Alcalase）的水解程度最高。“CH-Alcalase”和“CH-Alcalase<3 kDa”级分均表现出相当高的抗氧化活性和胶原酶抑制活性。因此，这些生物活性物质有可能从动物副产品中开发成为食品、化妆品和制药行业的抗氧化剂和抗衰老成分。
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