Botanical Institute 1, University of Karlsruhe, Kaiserstrasse 2, D-76128 Karlsruhe, Germany.
Plant J. 2010 Jun 1;62(5):829-39. doi: 10.1111/j.1365-313X.2010.04195.x. Epub 2010 Mar 2.
A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2, alpha-tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus-end marker GFP-EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell-to-cell movement of TMV encoding GFP-tagged movement protein (MP-GFP) is reduced in ATER2 accompanied by a reduced association of MP-GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.
七位来自 SR1 烟草突变体的小组（ATER1 到 ATER7）通过 T-DNA 激活标记和筛选对微管组装抑制剂乙基苯基氨基甲酸酯的抗性进行了筛选，用于研究微管在烟草花叶病毒（TMV）感染和传播过程中的作用。在其中一条线ATER2 中，α-微管蛋白从酪氨酸化形式转移到去酪氨酸化形式，并且微管正端标记 GFP-EB1 在 ATER2 突变体背景中表达时移动速度明显慢于 SR1 野生型。与 SR1 野生型相比，GFP 标记的运动蛋白（MP-GFP）编码 TMV 的细胞间运动效率在 ATER2 中降低，并且 MP-GFP 与胞间连丝的结合减少。与 SR1 野生型相比，这种突变体对病毒感染也更耐受，这意味着降低微管动力学在面对 TMV 感染时赋予了相对优势。
