Marx A M, Gressner A M
Department of Clinical Chemistry, Phillipps University, Marburg, Federal Republic of Germany.
J Clin Lab Anal. 1991;5(2):86-9. doi: 10.1002/jcla.1860050203.
We evaluated the analytical performance of two clinical chemistry slides developed recently by Eastman Kodak Company for the analysis of lactate and iron with the Kodak Ektachem 700 XR analyzer. Tested with supplemented serum pool materials we found imprecisions of the lactate analysis between 0.7% and 1.3% (within-run), resp., 1.0% and 1.5% (between-run). The imprecision of the iron analysis was between 1.0% and 2.2%, resp., 1.2% and 2.7%. Linearity of the methods was proven between 0.7 mmol/L and 13.0 mmol/L (lactate analysis) and between 5.4 mumol/L and 82.4 mummol/L (iron analysis), respectively. We found no significant interferences by hemolysis, lipemia, or bilirubin. The Kodak lactate results (x) correlated well with those of enzymatic methods (y) of Boehringer Mannheim (r = 0.995; y = 1.095x - 0.128) and of DuPont (r = 0.990; y = 1.015x - 0.242). The Kodak iron analysis (x) was correlated with the FerroZine methods (y) of Boehringer Mannheim (r = 0.988; y = 0.868x + 1.111), Hoffman LaRoche (r = 0.989; y = 0.868x + 3.416). and with atomic absorption (y) (r = 0.960; y = 0.826x + 3.416). We conclude that the newly developed methods for the analysis of lactate and iron with the Kodak Ektachem analyzer are acceptable alternatives to other common methods.
我们使用柯达Ektachem 700 XR分析仪评估了伊士曼柯达公司最近开发的两款临床化学载玻片在分析乳酸和铁方面的分析性能。使用添加血清混合材料进行测试时，我们发现乳酸分析的批内不精密度在0.7%至1.3%之间，批间不精密度在1.0%至1.5%之间。铁分析的不精密度分别在1.0%至2.2%和1.2%至2.7%之间。该方法的线性在0.7 mmol/L至13.0 mmol/L（乳酸分析）和5.4 μmol/L至82.4 μmol/L（铁分析）之间得到了验证。我们发现溶血、脂血或胆红素均无显著干扰。柯达乳酸结果（x）与勃林格曼海姆公司的酶法结果（y）相关性良好（r = 0.995；y = 1.095x - 0.128）以及杜邦公司的结果（r = 0.990；y = 1.015x - 0.242）。柯达铁分析（x）与勃林格曼海姆公司的FerroZine方法（y）（r = 0.988；y = 0.868x + 1.111）、霍夫曼 - 罗氏公司的方法（r = 0.989；y = 0.868x + 3.416）以及原子吸收法（y）（r = 0.960；y = 0.826x + 3.416）相关。我们得出结论，使用柯达Ektachem分析仪分析乳酸和铁的新开发方法是其他常用方法的可接受替代方法。
