Izumida Y
2nd Department of Physiology, School of Medicine, Ehime University, Japan.
Nihon Seirigaku Zasshi. 1991;53(1):1-12.
The effect of plasma proteins (and IgG fragments) and sialic acid content of erythrocytes on the aggregation of human erythrocytes was quantitatively examined by using a rheoscope combined with a television image analyser and a computer. (1) The velocity of erythrocyte aggregation by plasma proteins was increased with increasing in their molecular weight, i.e., IgG less than IgA less than fibrinogen less than IgM. F(ab')2. Fab and Fc could not induce the aggregation. (2) The aggregation induced by fibrinogen was accelerated by IgG and its peptic fragment, F(ab')2, but was unaffected by the plasmic fragments, Fab and Fc. The accelerating effect by IgG and F(ab')2 was inhibited by Fab and Fc. (3) The aggregation of erythrocytes was accelerated by decreasing the sialic acid content (due to the reduction of the electrostatic repulsive force among erythrocytes), and the effect of desialylation on the IgG-induced aggregation was greater than that of desialylation on the fibrinogen-induced aggregation. (4) The roles of plasma proteins and of sialic acid content of erythrocytes on the aggregation of erythrocytes were discussed.
使用流变仪结合电视图像分析仪和计算机，定量研究了血浆蛋白（及IgG片段）和红细胞唾液酸含量对人红细胞聚集的影响。（1）血浆蛋白诱导红细胞聚集的速度随分子量增加而加快，即IgG＜IgA＜纤维蛋白原＜IgM。F(ab')2、Fab和Fc不能诱导聚集。（2）纤维蛋白原诱导的聚集被IgG及其胃蛋白酶片段F(ab')2加速，但不受血浆片段Fab和Fc影响。IgG和F(ab')2的加速作用被Fab和Fc抑制。（3）红细胞聚集因唾液酸含量降低（由于红细胞间静电斥力减小）而加速，去唾液酸化对IgG诱导聚集的作用大于对纤维蛋白原诱导聚集的作用。（4）讨论了血浆蛋白和红细胞唾液酸含量在红细胞聚集中的作用。
