Izumida Y, Seiyama A, Maeda N
Department of Physiology, School of Medicine, Ehime University Shigenobu, Japan.
Biochim Biophys Acta. 1991 Aug 26;1067(2):221-6. doi: 10.1016/0005-2736(91)90047-c.
Relation between aggregating force (of fibrinogen and IgG) and disaggregating force (due to electrostatic repulsion among erythrocytes) in erythrocyte aggregation was investigated with a rheoscope combining a video camera, an image analyzer and a computer. (i) Erythrocyte aggregation was augmented with the increase of molecular weight of bridging macromolecules as far as examined for fibrinogen and the degradation products and IgG and the related macromolecules, and the augmentation seemed to be dependent on the molecular length of macromolecules. In accelerating the erythrocyte aggregation, fibrinogen was more effective than IgG, and some interaction between fibrinogen and IgG in their coexistence was suggested. (ii) The decrease of sialic acid content on the erythrocyte surface accelerated IgG-induced erythrocyte aggregation much greater than fibrinogen-induced one. (iii) Counteraction between aggregating force and disaggregating force in leading to erythrocyte aggregation was discussed relating to molecular length of bridging macromolecule and electrostatic repulsive force by sialic acid.
利用一台结合了摄像机、图像分析仪和计算机的血流仪，研究了红细胞聚集中（纤维蛋白原和IgG的）聚集力与（由于红细胞间静电排斥产生的）解聚力之间的关系。（i）就纤维蛋白原及其降解产物、IgG及相关大分子而言，在所研究的范围内，随着桥连大分子分子量的增加，红细胞聚集增强，且这种增强似乎取决于大分子的分子长度。在加速红细胞聚集方面，纤维蛋白原比IgG更有效，提示纤维蛋白原和IgG共存时存在某种相互作用。（ii）红细胞表面唾液酸含量的降低对IgG诱导的红细胞聚集的加速作用远大于对纤维蛋白原诱导的红细胞聚集的加速作用。（iii）结合桥连大分子的分子长度和唾液酸产生的静电排斥力，讨论了聚集力和解聚力在导致红细胞聚集中的对抗作用。
