Ceuta L O, Boehs G, Santos J J B
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brazil.
Braz J Biol. 2010 Feb;70(1):125-7. doi: 10.1590/s1519-69842010000100017.
The samples of Tagelus plebeius and Iphigenia brasiliana were manually collected on the Cachoeira River estuary region (Ilhéus, BA, Brazil) between August 2005 and August 2006, with a periodicity of 15 days, with 20 animals collected/sampled, performing 500 samples from each species. The animals were measured, eviscerated and kept in solution of Davidson and after 24-30 hours, they were transferred to ethanol 70%. The material was processed for routine histology, with paraffin embedding, obtaining 7 microm thick slices, stained with Harris hematoxilin and Eosin (HE). By light microscopy analysis, 2 cases of hermaphroditism (0.4%) in T. plebeius samples and one case (0.2%) in I. brasiliana were registered with predominance of female over male follicles.
2005年8月至2006年8月期间，在卡舒埃拉河河口地区（巴西巴伊亚州伊列乌斯）以15天为周期，人工采集了塔氏蛤蜊（Tagelus plebeius）和巴西伊菲姬娅蛤（Iphigenia brasiliana）的样本，每次采集/取样20只动物，每个物种共采集500个样本。对动物进行测量、解剖，保存在戴维森固定液中，24 - 30小时后转移至70%乙醇中。材料经石蜡包埋等常规组织学处理，制成7微米厚的切片，用苏木精 - 伊红（HE）染色。通过光学显微镜分析，在塔氏蛤蜊样本中记录到2例雌雄同体（0.4%），在巴西伊菲姬娅蛤中记录到1例（0.2%），均以雌性卵泡为主。
