Böckelmann P K, Ochandio B S, Bechara I J
Laboratório de Histofisiologia e Histopatologia Experimental em Ectotérmicos, Departamento de Histologia e Embriologia, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Braz J Biol. 2010 Feb;70(1):217-23. doi: 10.1590/s1519-69842010000100030.
Teleostean fins when partially amputated suffer a regenerative process called epimorphic regeneration, characterized by the following stages: healing, based on the formation of a multistratified epidermal layer, the formation of a mass of pluripotent cells known as blastema, the differentiation of these cells, the synthesis and disposition of the extracellular matrix, morphological growth and restoration. The epidermis has a fundamental role in the regenerative process of fish fins, as the healing time of this structure leads it to a faster regenerative process and it also works as a defense against the external environment. In this sense, due to the fast regeneration shown by the epidermis, the aim of this paper is to study the histology of the regenerative dynamics of the carp fin tail (Cyprinus carpio), under the light and transmission electron microscope. Epidermic regeneration begins right in the first hours after the fin amputation and it continues throughout the regenerative process. After 24 hours, an apical epidermal cap is established. Cytoplasmatic prolongations and intercellular junctions are observed and the cells of the basal layer of the epidermis change from the cubic form to the cylindrical, due to the development of the cytoplasmatic organelles responsible for the synthesis of the basal membrane, lost after amputation. These results show the importance of histological studies in regenerative processes. We believe that the association of molecular biology with histological studies can throw further light onto these regenerative dynamics.
硬骨鱼的鳍在部分截肢后会经历一个称为形态再生的再生过程，其特征如下阶段：愈合，基于多层表皮层的形成；多能细胞团即芽基的形成；这些细胞的分化；细胞外基质的合成与分布；形态生长和恢复。表皮在鱼鳍的再生过程中起着至关重要的作用，因为该结构的愈合时间使其再生过程更快，并且它还起到抵御外部环境的作用。从这个意义上说，由于表皮显示出快速再生，本文旨在在光学显微镜和透射电子显微镜下研究鲤鱼尾鳍（鲤）再生动力学的组织学。表皮再生在鳍截肢后的最初几个小时就开始了，并在整个再生过程中持续进行。24小时后，形成顶端表皮帽。观察到细胞质延长和细胞间连接，并且由于负责合成截肢后丢失的基底膜的细胞质细胞器的发育，表皮基底层的细胞从立方形态变为圆柱形态。这些结果表明了组织学研究在再生过程中的重要性。我们认为分子生物学与组织学研究的结合可以进一步阐明这些再生动力学。
