Université de Lyon, France.
Ann N Y Acad Sci. 2010 Feb;1189:113-20. doi: 10.1111/j.1749-6632.2009.05176.x.
Until recently, monitoring of cells and cellular activities at high hydrostatic pressure (HHP) was mainly limited to ex situ observations. Samples were analyzed prior to and following the depressurization step to evaluate the effect of the pressure treatment. Such ex situ measurements have several drawbacks: (i) it does not allow for kinetic measurements and (ii) the depressurization step often leads to artifactual measurements. Here, we describe recent advances in diamond anvil cell (DAC) technology to adapt it to the monitoring of microbial processes in situ. The modified DAC is asymmetrical, with a single anvil and a diamond window to improve imaging quality and signal collection. Using this novel DAC combined to Raman and X-ray spectroscopy, we monitored the metabolism of glucose by baker's yeast and the reduction of selenite by Agrobacterium tumefaciens in situ under HHP. In situ spectroscopy is also a promising tool to study piezophilic microorganisms.
直到最近，在高压（HHP）下对细胞和细胞活动的监测主要局限于非原位观察。在减压步骤之前和之后对样品进行分析，以评估压力处理的效果。这种非原位测量有几个缺点：（i）它不允许进行动力学测量，（ii）减压步骤通常会导致人为的测量。在这里，我们描述了金刚石压腔（DAC）技术的最新进展，以适应原位监测微生物过程。改进后的 DAC 是不对称的，只有一个砧座和一个金刚石窗口，以提高成像质量和信号收集。使用这种新型 DAC 结合拉曼和 X 射线光谱学，我们原位监测了面包酵母的葡萄糖代谢和 Agrobacterium tumefaciens 对亚硒酸盐的还原在 HHP 下。原位光谱学也是研究嗜压微生物的有前途的工具。
