P-糖蛋白和多药耐药相关蛋白的表达与胃癌的多药耐药相关。

Expression of P-glycoprotein and multidrug resistance-associated protein is associated with multidrug resistance in gastric cancer.

作者信息

Xu H-W, Xu L, Hao J-H, Qin C-Y, Liu H

机构信息

Department of Gastroenterology, Provincial Hospital Affiliated to Shandong University, Jinan, China.

出版信息

J Int Med Res. 2010 Jan-Feb;38(1):34-42. doi: 10.1177/147323001003800104.

DOI:10.1177/147323001003800104

PMID:20233511

Abstract

This study evaluated the sensitivities of gastric cancer cells to various chemotherapy drugs, and investigated the relationship between the expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) and multidrug resistance. Drug sensitivities were determined using a methyltetrazolium assay: expression levels of P-gp and MRP were measured using immunohistochemistry. On purification culture, gastric cancer cells were found to be most sensitive to cisplatin, mitomycin and adriamycin, moderately sensitive to etoposide and 5-fluorouracil, and less sensitive to homocamptothecin and methotrexate, with sensitivities of 76.7%, 70.0%, 66.7%, 60.0%, 56.7%, 43.3% and 30.0%, respectively. Positive expression for P-gp and MRP in gastric cancer tissues was 41.7% and 29.2%, respectively; coexpression of P-gp and MRP in cancer tissue was 23%. The drug-resistant groups had higher positive expression of P-gp and MRP compared with the drug-sensitive groups. In conclusion, expression of P-gp and MRP seems to be associated with multidrug resistance in gastric cancer.

摘要

本研究评估了胃癌细胞对多种化疗药物的敏感性，并探讨了P-糖蛋白（P-gp）和多药耐药相关蛋白（MRP）的表达与多药耐药之间的关系。采用甲基四氮唑法测定药物敏感性；应用免疫组织化学法检测P-gp和MRP的表达水平。纯化培养时发现，胃癌细胞对顺铂、丝裂霉素和阿霉素最为敏感，对依托泊苷和5-氟尿嘧啶中度敏感，对羟基喜树碱和甲氨蝶呤较不敏感，其敏感性分别为76.7%、70.0%、66.7%、60.0%、56.7%、43.3%和30.0%。胃癌组织中P-gp和MRP的阳性表达率分别为41.7%和29.2%；癌组织中P-gp和MRP的共表达率为23%。与药物敏感组相比，耐药组P-gp和MRP的阳性表达更高。总之，P-gp和MRP的表达似乎与胃癌的多药耐药有关。