[从脑组织中分离磷酸肌醇组分的制备方法]
[Preparative method of isolating phosphoinositide fractions from brain tissue].
作者信息
Kiselev G V
出版信息
Biokhimiia. 1977 Dec;42(12):2202-5.
A simple technique for preparative isolation of chromatographically homogenous fractions of mono-, di- and triphosphoinositides from ox brain tissue is described. Podyphosphoinositides fractions were obtained after chromatography of lipid extract on DEAE cellulose, phosphomonoinositides fraction--after chromatography of polyphosphoinositide-free material on aluminium hydroxide column. Bivalent metal ions were eliminated from lipid extract using chromatography on Dowex-50 H+. Ammonium acetate was removed after precipitation of lipids in water: methanol (1:1) in the presence of 4 M this salt. The average yield of mono-, di- and triphosphates was 40, 22 and 58 mg respectively per 1 kg of brain tissue as callulated for lipid phosphorus.
本文描述了一种从牛脑组织中制备分离单磷酸、二磷酸和三磷酸肌醇色谱纯级分的简单技术。脂质提取物经DEAE纤维素柱色谱后得到多磷酸肌醇级分，不含多磷酸肌醇的物质经氢氧化铝柱色谱后得到磷酸单肌醇级分。脂质提取物通过Dowex - 50 H⁺柱色谱去除二价金属离子。在4 M该盐存在下，脂质在水：甲醇（1:1）中沉淀后除去乙酸铵。以脂质磷计算，每1 kg脑组织中单磷酸、二磷酸和三磷酸的平均产量分别为40、22和58 mg。
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