肾综合征出血热患者血浆TH1/TH2细胞因子水平的变化

[Change of plasma TH1/TH2 cytokines levels in patients with hemorrhagic fever with renal syndrome].

作者信息

Dai Yi, Li Xu-li, Wang Lu, Qiu Zhi-feng, Li Tai-sheng

机构信息

Department of Infectious Diseases, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2010 Feb;32(1):108-12. doi: 10.3881/j.issn.1000-503X.2010.01.024.

DOI:10.3881/j.issn.1000-503X.2010.01.024

PMID:20236599

Abstract

OBJECTIVE

To observe changes in T cell subsets and TH1/TH2 secreted cytokines in the plasma of patients with hemorrhagic fever with renal syndrome (HFRS).

METHODS

Totally 22 patients with HFRS (9 mild cases and 13 moderate cases) were enrolled. Blood samples were taken 1, 4, and 12 weeks after presentation. T cell subsets were tested by flow cytometry (FCM), and the expression of cytokines in plasma were analysed with enzyme-linked immunosorbent assay (ELISA). Another 16 healthy blood donors were enrolled as the control group.

RESULTS

CD3 + CD8 + T lymphocytes increased at week 1 and 4 (P < 0.01), which was more significant in mild cases than in moderate cases (P < 0.05). The change of CD3 + CD4 + T lymphocytes during the disease course were not significantly different from that in control group (P > 0.05). One week after presentation, TH1 [interleukin (IL)-2 and interferon-gamma (IFN-gamma)] and TH2 (IL-6, IL-10) cytokine productions were significantly higher in HFRS patients than in the control group (P < 0.01); IL-2 and IL-10 remained high levels during the whole observation period, and were still significantly higher than in the control group (P < 0.01). At week 4, the plasma IL-5 level was significantly higher in HFRS patients than in the control group (P < 0.01), and were still significantly higher than in the control group at week 12 (P < 0.01). At week 1 and 4, the plasma INF-gamma levels were significantly higher in moderate patients than in mild patients (P < 0.05); at week 12, the plasma IL-10 level was significantly higher in moderate patients than in mild patients(P < 0.05).

CONCLUSIONS

CD3 + CD4 + T lymphocytes remarkably increases at the early stage of disease in patients with mild HFRS. The early cell mediated immune response is helpful for disease control. The cytokines INF-gamma and IL-10 increase more obviously in moderate patients, indicating that cytokines also are key pathogenic factors of HRFS.

摘要

目的

观察肾综合征出血热（HFRS）患者血浆T细胞亚群及TH1/TH2分泌细胞因子的变化。

方法

纳入22例HFRS患者（9例轻症患者和13例中症患者）。在患者就诊后第1、4和12周采集血样。采用流式细胞术（FCM）检测T细胞亚群，并用酶联免疫吸附测定（ELISA）分析血浆中细胞因子的表达。另纳入16名健康献血者作为对照组。

结果

CD3 + CD8 + T淋巴细胞在第1周和第4周增加（P < 0.01），轻症患者比中症患者更显著（P < 0.05）。疾病过程中CD3 + CD4 + T淋巴细胞的变化与对照组无显著差异（P > 0.05）。就诊后1周，HFRS患者的TH1 [白细胞介素（IL）-2和干扰素-γ（IFN-γ）]和TH2（IL-6、IL-10）细胞因子产生显著高于对照组（P < 0.01）；IL-2和IL-10在整个观察期内保持高水平，仍显著高于对照组（P < 0.01）。在第4周，HFRS患者血浆IL-5水平显著高于对照组（P < 0.01），在第12周仍显著高于对照组（P < 0.01）。在第1周和第4周，中症患者血浆INF-γ水平显著高于轻症患者（P < 0.05）；在第12周，中症患者血浆IL-10水平显著高于轻症患者（P < 0.05）。