母体白细胞介素-6（-174）C/C 多态性与早产儿的绒毛膜羊膜炎和囊性脑室周围白质软化症有关。

Maternal interleukin-6 (-174) C/C polymorphism is associated with chorioamnionitis and cystic periventricular leucomalacia of the preterm infant.

机构信息

Research Unit for Neonatal Infectious Diseases and Epidemiology, Division of Neonatology, Department of Pediatrics, Medical University of Graz, Graz, Austria.

出版信息

J Perinatol. 2010 Nov;30(11):712-6. doi: 10.1038/jp.2010.30. Epub 2010 Mar 18.

DOI:10.1038/jp.2010.30

PMID:20237486

Abstract

OBJECTIVE

To evaluate the association between maternal interleukin (IL)-6 G(-174)C polymorphism and cystic periventricular leukomalacia (cPVL) of the preterm newborn.

STUDY DESIGN

After searching a local database, we recruited 132 preterm infants with diagnosis of cPVL, 44 Caucasian mothers were also recruited to participate in this candidate gene-association study at a single teritary care center. Data related to maternal IL-6 G(-174)C polymorphisms were compared with 41 controls, and furthermore compared with data from umbilical cord blood samples from a consecutive birth cohort of 395 healthy newborns, and published data from Caucasian populations including 1104 adults, respectively. In addition, subgroup analysis was performed in cases with either history of preterm premature rupture of the membranes (PPROM) or clinical chorioamnionitis (CCA). IL-6 genotyping was performed using an allele-specific polymerase chain reaction technique.

RESULT

Frequencies of the IL-6 G(-174)C polymorphisms did not differ between cases (GG, 29.5%; GC, 54.5% and CC, 15.9%) and controls (GG, 34.2; GC, 51.2 and CC, 14.6%). Subgroup analysis of 31 cases with history of PPROM (GG, 25.8; GC, 54.8 and CC 19.4%) and controls did not reveal significant differences, but a significantly higher frequency of the CC genotype was found in 23 cases with a history of CCA (34.8%) compared with controls by either univariate (P=0.032; odds ratio 3.11, 95% confidence interval (CI) 1.11 to 8.68) or multivariate analysis (P=0.049, odds ratio 2.54, 95% CI 1.01 to 6.45). These data were confirmed by a comparing the CC genotype frequency to 395 term controls (CC 14.7%, P=0.005) and to the mean CC genotype frequency of 1104 Caucasian adults (CC 15.6%, P<0.0001).

CONCLUSION

Frequencies of the IL-6 G(-174)C polymorphisms did not differ between groups. Subgroup analysis revealed an association of the CC genotype with CCA and cPVL in the preterm newborn.

摘要

目的

评估母体白细胞介素（IL）-6 G（-174）C 多态性与早产儿囊性脑室周围白质软化症（cPVL）之间的关联。

研究设计

在搜索本地数据库后，我们招募了 132 名诊断为 cPVL 的早产儿，还招募了 44 名白人母亲在一家单一的三级护理中心参与候选基因关联研究。将与母体 IL-6 G（-174）C 多态性相关的数据与 41 名对照者进行比较，并进一步与 395 名健康新生儿连续出生队列的脐带血样本数据以及包括 1104 名成年人在内的白人人群的已发表数据进行比较。此外，在有早产胎膜早破（PPROM）病史或临床绒毛膜羊膜炎（CCA）的病例中进行了亚组分析。采用等位基因特异性聚合酶链反应技术对 IL-6 基因进行分型。

结果

病例组（GG，29.5%；GC，54.5%和 CC，15.9%）和对照组（GG，34.2%；GC，51.2%和 CC，14.6%）中 IL-6 G（-174）C 多态性的频率没有差异。31 例有 PPROM 病史的亚组分析（GG，25.8%；GC，54.8%和 CC 19.4%）和对照组未见明显差异，但有 CCA 病史的 23 例 CC 基因型频率明显高于对照组，单因素分析（P=0.032；优势比 3.11，95%置信区间（CI）1.11 至 8.68）或多因素分析（P=0.049，优势比 2.54，95%CI 1.01 至 6.45）。通过将 CC 基因型频率与 395 名足月对照组（CC 14.7%，P=0.005）和 1104 名白种成年人的平均 CC 基因型频率（CC 15.6%，P<0.0001）进行比较，证实了这些数据。