The National Key Facilities for Crop Gene Resources and Genetic Improvement, NFCRI, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
Genome. 2010 Mar;53(3):241-9. doi: 10.1139/g09-098.
The St, P, and Y genomes are three important genomes in the tribe Triticeae, which includes many perennial species. To study polymorphisms within the chromosomes of the St, P, and Y genomes, a GISH-FISH method was developed that allowed them to be clearly distinguished. The karyotypes of five individuals from population Z1925 of Kengyilia grandiglumis (Keng) J.L. Yang et al. (2n = 6x = 42, StStPPYY) were analyzed. The results showed that there were structural polymorphisms in all of the chromosomes from the three individual genomes. The polymorphisms were found mainly in the terminal regions of chromosomes and infrequently near the centromeric region. Of all the chromosomes, 1P, 1St, 1Y, 2Y, 3St, and 3Y showed the most polymorphisms. The polymorphisms within the individual chromosomes suggested that more extensive and scientific conclusions regarding the origin and evolution of genomes in wild species of Triticeae would be achieved by studying a population as a sampling and analysis unit.
St、P 和 Y 基因组是包含许多多年生物种的小麦族中的三个重要基因组。为了研究 St、P 和 Y 基因组中染色体的多态性,开发了一种 GISH-FISH 方法,可以清楚地区分它们。对来自节节麦(Kengyilia grandiglumis (Keng) J.L. Yang et al.)Z1925 群体的五个个体的核型进行了分析。结果表明,来自三个个体基因组的所有染色体都存在结构多态性。多态性主要存在于染色体的末端区域,很少存在于着丝粒区域附近。在所有染色体中,1P、1St、1Y、2Y、3St 和 3Y 显示出最多的多态性。个体染色体内的多态性表明,通过将种群作为采样和分析单位进行研究,将能够更广泛和科学地得出关于小麦族野生种基因组起源和进化的结论。