He Fang, Xing Piyi, Bao Yinguang, Ren Mingjian, Liu Shubing, Wang Yuhai, Li Xingfeng, Wang Honggang
Guizhou Subcenter of National Wheat Improvement Center, College of Agronomy, Guizhou University, Guiyang, China.
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, China.
Front Plant Sci. 2017 Dec 19;8:2161. doi: 10.3389/fpls.2017.02161. eCollection 2017.
In this study, the intergeneric hybrids F, F, BCF, BCF, and BCF from and crosses were produced to study their chromosome pairing behavior. The average chromosome configuration of the two F hybrids agreed with the theoretical chromosome configuration of 21I+7II, indicating that the genomic constitution of this F hybrid was ABDStStEEE. Compared with the BCF generation, the BCF generation showed a rapid decrease in the number of chromosomes and the BCF generation showed a more extensive distribution of chromosomes. In addition, pairing between wheat and chromosomes was detected in each of the wheat hybrid progenies, albeit rarely. Our results demonstrated that genomic hybridization (GISH) using an genomic DNA probe offers a reliable approach for characterizing chromosome pairing in wheat and hybrid progenies.
在本研究中，通过 和 的杂交产生了属间杂种F、F、BCF、BCF和BCF，以研究它们的染色体配对行为。两个F杂种的平均染色体构型与理论染色体构型21I+7II相符，表明该F杂种的基因组组成为ABDStStEEE。与BCF代相比，BCF代的 染色体数量迅速减少，而BCF代的 染色体分布更广泛。此外，在每个小麦杂交后代中均检测到小麦与 染色体之间的配对，尽管频率很低。我们的结果表明，使用 基因组DNA探针的基因组原位杂交（GISH）为表征小麦和 杂交后代的染色体配对提供了一种可靠的方法。
