Gilkes N R, Weeks G
Can J Biochem. 1977 Dec;55(12):1233-6. doi: 10.1139/o77-184.
A novel procedure was recently described for the purification of plasma membranes of Dictyostelium discoideum (Gilkes, N. R. & Weeks, G. (1977) Biochim. Biophys. Acta 464, 142-156). Considerable enrichment of plasma membrane marker enzymes was achieved, but since purified mitochondrial and endoplasmic reticulum fractions were unavailable, it was not possible to accurately assess the contamination level of these organelles. We have therefore slightly modified the plasma membrane preparation procedure, improving purification, and have prepared partially purified mitochondrial and endoplasmic reticulum fractions. The data suggest that the contamination of the plasma membranes by endoplasmic reticulum membranes is no greater than 10%, and probably considerably less. No mitochondrial contamination is detectable.
最近描述了一种用于纯化盘基网柄菌质膜的新方法（吉尔克斯，N. R. & 威克斯，G.（1977年）《生物化学与生物物理学报》464卷，第142 - 156页）。实现了质膜标记酶的显著富集，但由于无法获得纯化的线粒体和内质网组分，因此无法准确评估这些细胞器的污染水平。因此，我们对质膜制备方法进行了轻微修改，提高了纯化程度，并制备了部分纯化的线粒体和内质网组分。数据表明，内质网对质膜的污染不超过10%，可能远低于此。未检测到线粒体污染。
