新合成的组蛋白与复制型SV40 DNA的优先结合。

Preferential association of newly synthesized histones with replicating SV40 DNA.

作者信息

Crémisi C, Chestier A, Yaniv M

出版信息

Cell. 1977 Dec;12(4):947-51. doi: 10.1016/0092-8674(77)90159-3.

PMID:202394

Abstract

The assembly of newly synthesized histones into nucleosomes during replication of SV40 minichromosomes in vivo was studied. Infected cells were labeled with 35S-methionine for a time shorter than that required to complete a round of viral DNA replication. Mature and replicating SV40 minichromosomes were extracted and separated by zonal sedimentation, and their histone content was analyzed by polyacrylamide gel electrophoresis (SDS and acidic urea). We show that the pulse-labeled histones associate preferentially with the replicating DNA.

摘要

我们研究了在体内SV40微型染色体复制过程中，新合成的组蛋白组装成核小体的情况。用35S-甲硫氨酸标记感染细胞的时间，短于完成一轮病毒DNA复制所需的时间。提取成熟的和正在复制的SV40微型染色体，并通过区带沉降进行分离，然后通过聚丙烯酰胺凝胶电泳（SDS和酸性尿素）分析它们的组蛋白含量。我们发现，脉冲标记的组蛋白优先与正在复制的DNA结合。