组蛋白缺陷型SV40染色质的复制活性。
Replicative activity of histone-deficient SV40 chromatin.
作者信息
Green M H, Brooks T L
出版信息
Nucleic Acids Res. 1979 Nov 24;7(6):1687-98. doi: 10.1093/nar/7.6.1687.
Histone-deficient SV40 chromatin, selectively radiolabeled in the DNA following the addition of cycloheximide to infected monkey cells, was compared with the normal 55S viral chromatin for its ability to serve as a template for a subsequent round of replication. After the removal of cycloheximide, the 26S histone-deficient SV40 chromatin was converted to apparently normal 55S chromatin. During this conversion, the chromatin which sedimented at 26-40S failed to replicate whereas the 44-55S chromatin contained a large fraction (28%) of newly replicated DNA molecules. Thus, the DNA in the 26S histone-deficient 40S chromatin cannot replicate without the prior and/or concommitant addition of protein which increases its sedimentation rate to 41-55S. Nevertheless, when compared with normal 55S viral chromatin, the histone-deficient SV40 chromatin had nearly a 3-fold greater probability of functioning as a template for a subsequent round of replication.
在感染的猴细胞中加入环己酰亚胺后，对DNA进行选择性放射性标记的组蛋白缺陷型SV40染色质，与正常的55S病毒染色质作为后续一轮复制模板的能力进行了比较。去除环己酰亚胺后，26S组蛋白缺陷型SV40染色质转化为明显正常的55S染色质。在这种转化过程中，沉降在26 - 40S的染色质无法复制，而44 - 55S染色质含有很大一部分（28%）新复制的DNA分子。因此，26S组蛋白缺陷型40S染色质中的DNA在没有预先和/或同时添加能将其沉降速率提高到41 - 55S的蛋白质的情况下无法复制。然而，与正常的55S病毒染色质相比，组蛋白缺陷型SV40染色质作为后续一轮复制模板发挥作用的概率几乎高3倍。
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