结节拟杆菌强毒株和弱毒株的特性分析
Characterisation of virulent and benign strains of Bacteroides nodosus.
作者信息
Depiazzi L J, Richards R B, Henderson J, Rood J I, Palmer M, Penhale W J
机构信息
Regional Veterinary Laboratory, Department of Agriculture, Bunbury, Australia.
出版信息
Vet Microbiol. 1991 Jan;26(1-2):151-60. doi: 10.1016/0378-1135(91)90051-g.
The extracellular proteases of 395 isolates of B. nodosus from ovine, bovine and caprine foot lesions were classified as either thermostable or thermolabile. Stable protease was associated with one and unstable protease with four distinctive isoenzyme patterns, each pattern differentiated by the relative mobility of paired isoenzymes. Pathogenicity tests on 64 isolates showed a correlation between the production of stable protease and the production of virulent ovine footrot lesions. The mean values for total protease activity, twitching motility and colony diameter were significantly higher for virulent compared to benign isolates, but the range of values overlapped. SDS-PAGE whole-cell electrophoretic profiles of virulent isolates were similar to the profiles of some benign isolates.
从绵羊、牛和山羊足部病变分离出的395株结节杆菌的细胞外蛋白酶被分类为热稳定型或热不稳定型。稳定型蛋白酶与一种模式相关，不稳定型蛋白酶与四种独特的同工酶模式相关，每种模式通过成对同工酶的相对迁移率来区分。对64株分离菌进行的致病性测试表明，稳定型蛋白酶的产生与绵羊恶性足腐病损伤的产生之间存在相关性。与良性分离菌相比，恶性分离菌的总蛋白酶活性、颤动运动性和菌落直径的平均值显著更高，但数值范围有重叠。恶性分离菌的SDS-PAGE全细胞电泳图谱与一些良性分离菌的图谱相似。
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