Kortt A A, Burns J E, Stewart D J
Res Vet Sci. 1983 Sep;35(2):171-4.
The extracellular proteases of Bacteroides nodosus were separated electrophoretically on polyacrylamide slab gels at pH 8 . 8. Proteolytic activity was detected by placing the gel slab on a layer of gelatin-agar and allowing hydrolysis of the gelatin to occur. After two hours at 37 degrees C, the unhydrolysed gelatin was precipitated with mercuric chloride in acid. The proteolytic zymograms of 14 benign strains were identical. The zymograms of 10 virulent strains yielded two closely related sets of proteases. The reproducible difference observed between the respective zymograms readily provides a rapid diagnostic test to assist in the identification of benign and virulent strains of B nodosus.
在pH 8.8的条件下，用聚丙烯酰胺平板凝胶电泳法分离结节拟杆菌的胞外蛋白酶。通过将凝胶平板置于一层明胶 - 琼脂上，使明胶发生水解来检测蛋白水解活性。在37℃下放置两小时后，用酸性氯化汞沉淀未水解的明胶。14株良性菌株的蛋白水解酶谱相同。10株强毒株的酶谱产生了两组密切相关的蛋白酶。在各自的酶谱之间观察到的可重复差异很容易提供一种快速诊断测试，以协助鉴定结节拟杆菌的良性和强毒株。
