Nobusawa E, Aoyama T, Kato H, Suzuki Y, Tateno Y, Nakajima K
Department of Microbiology, Institute of Public Health, Tokyo, Japan.
Virology. 1991 Jun;182(2):475-85. doi: 10.1016/0042-6822(91)90588-3.
We determined the sequences of 7 serotypes (H4, H6, H8, H9, H11, H12, and H13) of hemagglutinin (HA) genes, which have not been reported so far. The coding regions consisted of 1692 nucleotides in H4, 1698 in H6, 1695 in H8, 1680 in H9, 1695 in H11, 1692 in H12, and 1698 in H13, and specified 564, 566, 565, 560, 565, 564, and 566 amino acids, respectively. By comparison of amino acid sequences, 13 HA serotypes could be divided into two families, i.e., an H1 group (H1, H2, H5, H6, H8, H9, H11, H12, and H13) and an H3 group (H3, H4, H7, and H10). The relationship was essentially similar to that reported by Air from the comparison of 80 amino-terminal amino acid sequence of 12 HA serotypes (G.M. Air, 1981, Proc. Natl. Acad. Sci. USA 78, 7639-7643). Though a considerable amino acid sequence difference exists between certain HA serotypes, several amino acid residues in fusion peptides (HA2(1-11)) and receptor-binding sites (HA1(98), -134, -138, -153, -183, and -195) were shown to be conserved among the 13 HA serotypes. Human H1 and avian H3, H4, H8, and H10 viruses preferentially bound NeuAc alpha 2,3Gal sequences, whereas human H2 and H3 and avian H6 and H9 viruses bound NeuAc alpha 2,6Gal sequences, although the amino acid residues at position 226 of human H2 and avian H6 and H9 serotype HAs are glutamine. These results show that the amino acid residue at position 226 is not necessarily a determinant of receptor specificity for all serotypes.
我们测定了血凝素（HA）基因的7种血清型（H4、H6、H8、H9、H11、H12和H13）的序列，这些序列迄今尚未见报道。编码区在H4中由1692个核苷酸组成，H6中为1698个，H8中为1695个，H9中为1680个，H11中为1695个，H12中为1692个，H13中为1698个，分别编码564、566、565、560、565、564和566个氨基酸。通过氨基酸序列比较，13种HA血清型可分为两个家族，即H1组（H1、H2、H5、H6、H8、H9、H11、H12和H13）和H3组（H3、H4、H7和H10）。这种关系与Air通过比较12种HA血清型的80个氨基末端氨基酸序列所报道的基本相似（G.M. Air，1981，《美国国家科学院院刊》78，7639 - 7643）。尽管某些HA血清型之间存在相当大的氨基酸序列差异，但融合肽（HA2(1 - 11)）和受体结合位点（HA1(98)、-134、-138、-153、-183和-195）中的几个氨基酸残基在13种HA血清型中是保守的。人H1以及禽H3、H4、H8和H10病毒优先结合NeuAcα2,3Gal序列，而人H2和H3以及禽H6和H9病毒结合NeuAcα2,6Gal序列，尽管人H2以及禽H6和H9血清型HA的第226位氨基酸残基是谷氨酰胺。这些结果表明，第226位氨基酸残基不一定是所有血清型受体特异性的决定因素。
