Zimmermann K, Dobrovnik M, Ballaun C, Bevec D, Hauber J, Böhnlein E
SANDOZ Research Institute Vienna, Austria.
Virology. 1991 Jun;182(2):874-8. doi: 10.1016/0042-6822(91)90633-m.
Human immunodeficiency virus type 1 (HIV-1) gene expression is regulated by viral and cellular factors interacting with cis-elements located in the retroviral long terminal repeat (LTR). In this report we analyzed HIV-1 LTR-specific regulatory sequences responsive to the HIV-1 Tat and HTLV-I Tax trans-activator proteins. Our results indicate that the Sp1 binding sites in the HIV-1 LTR are crucially involved in Tat-mediated gene expression in human Jurkat T-cells whereas they are dispensable for HTLV-I Tax-induced activation. In contrast, the NF-kB binding sites within the HIV-1 LTR are essential for Tax-mediated transcription but had only marginal effect on Tat-induced reporter gene expression.
1型人类免疫缺陷病毒（HIV-1）的基因表达受病毒和细胞因子调控，这些因子与位于逆转录病毒长末端重复序列（LTR）中的顺式元件相互作用。在本报告中，我们分析了HIV-1 LTR特异性调控序列对HIV-1 Tat和HTLV-I Tax反式激活蛋白的反应。我们的结果表明，HIV-1 LTR中的Sp1结合位点在人类Jurkat T细胞中Tat介导的基因表达中起关键作用，而对于HTLV-I Tax诱导的激活则是可有可无的。相反，HIV-1 LTR内的NF-κB结合位点对于Tax介导的转录至关重要，但对Tat诱导的报告基因表达仅有微弱影响。
