Goretski J, Hollocher T C
Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Biochem Biophys Res Commun. 1991 Mar 29;175(3):901-5. doi: 10.1016/0006-291x(91)91650-2.
Two denitrifying bacteria, Pseudomonas stutzeri and Achromobacter cycloclastes, were incubated with Na15NO2 and NaN3 under conditions that allowed catalysis of nitrosyl transfer from nitrite to azide. This transfer, which is presumed to be mediated by the heme- and copper-containing nitrite reductase of P. stutzeri and A. cycloclastes, respectively, leads to formation of isotopically mixed 14,15N2O, whereas denitrification leads to 15N2O. The conditions that emphasized nitrosyl transfer also partially inhibited the nitric oxide reductase system and led to accumulation of 15NO. Absorption of NO from the gas phase by acidic CrSO4 in a sidewell largely abolished nitrosyl transfer to azide. With these two organisms, which are thought to be representative of denitrifiers generally, catalysis of nitrosyl transfer seemed to depend on NO.
将两种反硝化细菌，即施氏假单胞菌和环裂无色杆菌，与亚硝酸钠（Na15NO2）和叠氮化钠（NaN3）一起培养，培养条件要能促使亚硝酰基从亚硝酸盐转移至叠氮化物。这种转移，据推测分别由施氏假单胞菌和环裂无色杆菌中含血红素和铜的亚硝酸还原酶介导，会导致形成同位素混合的14,15N2O，而反硝化作用则会产生15N2O。强调亚硝酰基转移的条件也会部分抑制一氧化氮还原酶系统，并导致15NO的积累。侧孔中酸性硫酸铬（CrSO4）对气相中NO的吸收在很大程度上消除了亚硝酰基向叠氮化物的转移。对于这两种通常被认为是反硝化菌代表的微生物而言，亚硝酰基转移的催化作用似乎依赖于NO。
