Fernandes P L, Ferreira H G
Department of Cell Biology, Gulbenkian Institute of Science, Oeiras, Portugal.
Biochim Biophys Acta. 1991 Apr 26;1064(1):111-23. doi: 10.1016/0005-2736(91)90417-7.
A mathematical version of the cell model of the cortical thick ascending limb of the rabbit proposed by Greger and Schlatter ((1983) Pfügers Arch. 396, 325-334) is described. Available data are sufficient to compute the most important parameters. Simulations of experiments with perfused tubules in which the transepithelial voltage and conductance, and the intracellular electrical potential were measured in the course of ionic substitutions in the perfusing baths or treatment with ouabain or furosemide are in good agreement with the experimental results with the exception of those relating to dilution potential experiments. The model can be used in the analysis and planning of experiments and is capable of predicting the instantaneous values of ionic fluxes and intracellular concentrations and of cell volume.
描述了由格雷格和施拉特（(1983)《普弗吕格尔氏 Archiv》396, 325 - 334）提出的兔皮质厚升支细胞模型的数学版本。现有数据足以计算最重要的参数。对灌注小管实验的模拟，即在灌注浴中进行离子替代或用哇巴因或呋塞米处理过程中测量跨上皮电压、电导和细胞内电势，除了与稀释电位实验相关的结果外，与实验结果吻合良好。该模型可用于实验分析和规划，能够预测离子通量、细胞内浓度和细胞体积的瞬时值。
