Lomax C A, Bradley E, Weber J, Bourgaux P
Intervirology. 1978;9(1):28-38. doi: 10.1159/000148918.
Conditions necessary for the establishment and maintenance of transformation of human cells by wild type and temperature-sensitive mutants of SV40 were examined. For both early and late mutants, the frequency of transformation was found to be up to 5-fold higher, and virus yield 100-fold lower, at 39 degrees than at 33 degrees. No such effect was observed with the wild type virus under the same conditions. This observation is apparently at variance with previously published work, but may be explained by the semipermissive nature of the cells that we used. Increasing the temperature to 40.5 degrees caused cells transformed by the early mutant, tsA30, to lose T-antigen as detectable by staining, and also to lose the ability to grow to high density, while it produced no effect on cells transformed by wild type virus.
对野生型和温度敏感型SV40突变体建立和维持人类细胞转化所需的条件进行了研究。对于早期和晚期突变体，发现在39摄氏度时的转化频率比33摄氏度时高5倍，而病毒产量低100倍。在相同条件下，野生型病毒未观察到这种效应。这一观察结果显然与先前发表的工作不一致，但可能是由于我们所使用细胞的半允许性质所致。将温度提高到40.5摄氏度会导致由早期突变体tsA30转化的细胞通过染色检测不到T抗原，并且失去生长到高密度的能力，而对由野生型病毒转化的细胞没有影响。
