Rak J W, Basolo F, Elliott J W, Russo J, Miller F R
Department of Immunology, Michigan Cancer Foundation, Detroit 48201.
Cancer Lett. 1991 Apr;57(1):27-36. doi: 10.1016/0304-3835(91)90059-q.
Fluorescent lectin binding to cell surfaces was quantitatively analysed by flow cytometry on mortal human breast epithelial cells MCF-10M, the immortalized cell line MCF-10A derived from MCF-10M and sublines of MCF-10A transfected with the neomycin resistance gene (MCF-10Aneo), the c-Ha-ras protooncogene (MCF-10AneoN), or transfected and transformed with the c-Ha-ras activated oncogene (MCF-10AneoT). Immortal MCF-10A cells bound 10-fold more peanut agglutinin (PNA) and soy bean agglutinin (SBA) than did MCF-10M cells. Transformed MCF-10AneoT cells bound approximately ten times more PNA than did non-transformed cells transfected with protooncogene (MCF-10AneoN). Treatment of the transfectants with neuraminidase abrogated the differences in PNA-binding and reduced the differences in SBA binding. SDS-PAGE separation of PNA binding glycoproteins revealed different patterns for all MCF-10A derived sublines.
通过流式细胞术对人乳腺上皮 mortal 细胞 MCF-10M、源自 MCF-10M 的永生化细胞系 MCF-10A 以及用新霉素抗性基因（MCF-10Aneo）、c-Ha-ras 原癌基因（MCF-10AneoN）转染的 MCF-10A 亚系或用 c-Ha-ras 激活癌基因转染并转化的（MCF-10AneoT）细胞表面的荧光凝集素结合进行了定量分析。永生化的 MCF-10A 细胞比 MCF-10M 细胞结合的花生凝集素（PNA）和大豆凝集素（SBA）多 10 倍。转化的 MCF-10AneoT 细胞比用原癌基因转染的未转化细胞（MCF-10AneoN）结合的 PNA 大约多 10 倍。用神经氨酸酶处理转染细胞消除了 PNA 结合的差异，并减少了 SBA 结合的差异。PNA 结合糖蛋白的 SDS-PAGE 分离显示所有源自 MCF-10A 的亚系都有不同的模式。
