Green P H, Tall A R, Glickman R M
J Clin Invest. 1978 Feb;61(2):528-34. doi: 10.1172/JCI108963.
High density lipoprotein was isolated from pooled rat serum and mesenteric lymph of lymph fistula rats. In most experiments, 5,5'-dithionitrobenzoic acid, an inhibitor of the enzyme lecithin:cholesterol acyltransferase, was added during the collection of lymph to prevent modification of the lipid composition of newly secreted lipoproteins. Negative staining electron microscopy of lymph high density lipoprotein revealed discoidal particles (190+/-3 x 55+/-1 A) which tended to form rouleaux, smaller spherical particles were also present. Serum high density lipoprotein contained only spherical particles (diameter 93+/-4 A). Lipid analysis showed that lymph high density lipoprotein was enriched in phospholipid and deficient in cholesterol esters when compared to serum high density lipoprotein. The phospholipid to cholesterol esters ratio was greatest in basal lymph high density lipoprotein when compared to fatty lymph and serum high density lipoprotein. From analysis of the lipid compositional data and direct particle measurement by electron microscopy, it could be determined that congruent with50% of basal lymph high density lipoprotein and 30% of fatty lymph high density lipoprotein was discoid. Basal lymph high density lipoprotein was enriched in apoA-I and deficient in the arginine-rich peptide, and the apoprotein composition of fatty lymph high density lipoprotein more closely resembled serum. These observations demonstrate that intestinal lymph contains two types of high density lipoprotein particles, a discoid nascent particle deficient in cholesterol ester and rich in apoA-I, and spherical high density lipoprotein derived from plasma. A significant amount of lymph high density lipoprotein appears to be secreted by the intestine.
从大鼠混合血清和淋巴瘘大鼠的肠系膜淋巴中分离出高密度脂蛋白。在大多数实验中，在收集淋巴液时加入5,5'-二硫代硝基苯甲酸（一种卵磷脂：胆固醇酰基转移酶抑制剂），以防止新分泌的脂蛋白的脂质组成发生改变。淋巴高密度脂蛋白的负染色电子显微镜检查显示为盘状颗粒（190±3×55±1埃），倾向于形成缗钱状，也存在较小的球形颗粒。血清高密度脂蛋白仅包含球形颗粒（直径93±4埃）。脂质分析表明，与血清高密度脂蛋白相比，淋巴高密度脂蛋白富含磷脂且胆固醇酯含量不足。与脂肪淋巴和血清高密度脂蛋白相比，基础淋巴高密度脂蛋白中的磷脂与胆固醇酯的比例最大。通过对脂质组成数据的分析和电子显微镜直接测量颗粒，可以确定基础淋巴高密度脂蛋白的50%和脂肪淋巴高密度脂蛋白的30%与盘状颗粒一致。基础淋巴高密度脂蛋白富含载脂蛋白A-I且富含精氨酸的肽含量不足，脂肪淋巴高密度脂蛋白的载脂蛋白组成更类似于血清。这些观察结果表明，肠淋巴中含有两种类型的高密度脂蛋白颗粒，一种是胆固醇酯含量不足且富含载脂蛋白A-I的盘状新生颗粒，另一种是源自血浆的球形高密度脂蛋白。大量的淋巴高密度脂蛋白似乎是由肠道分泌的。
