State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Animal Sciences & Technology, Zhejiang A & F University, Hangzhou 311300, China.
Oxid Med Cell Longev. 2022 Jan 28;2022:2315575. doi: 10.1155/2022/2315575. eCollection 2022.
The liver is the center for uptake, synthesis, packaging, and secretion of lipids and lipoproteins. The research on lipid metabolism in pigs is limited. The objective of the present study is to identify the genes related to lipid metabolism and oxidative stress in pigs by using transcriptomic analysis. Liver segments were collected from 60 Jinhua pigs for the determination of liver lipid content. The 7 pigs with the highest and lowest liver lipid content were set as group H and group L, respectively. Liver segments and serum samples were collected from each pig of the H and L groups for RNA sequencing and the determination of triglycerides (TG) content and high-density lipoprotein cholesterol (HDL) content, respectively. The HDL content in the serum of pigs in the H group was significantly higher than the L group ( < 0.05). From transcriptomic sequencing, 6162 differentially expressed genes (DEGs) were identified, among which 2962 were upregulated and 3200 downregulated genes with the increase in the liver content of Jinhua pigs. After GO enrichment and KEGG analyses, lipid modification, cellular lipid metabolic process, cholesterol biosynthetic process, fatty acid metabolic process, oxidoreduction coenzyme metabolic process, oxidoreductase activity, acting on CH-OH group of donors, response to oxidative stress, nonalcoholic fatty liver disease (NAFLD), sphingolipid metabolism, and oxidative phosphorylation pathways were involved in lipid metabolism and oxidative stress in Jinhua pigs. For further validation, we selected 10 DEGs including 7 upregulated genes (, , , , , , and ) and 4 downregulated genes (, , and ) for RT-qPCR verification. To validate these results in other pig species, we analyzed these 10 DEGs in the liver of Duroc×Landrace×Yorkshire pigs. Similar expression patterns of these 10 DEGs were observed. These data would provide an insight to understand the gene functions regulating lipid metabolism and oxidative stress and would potentially provide theoretical basis for the development of strategies to modulate lipid metabolism and even control human diabetes and obesity by gene regulations.
肝脏是脂质和脂蛋白摄取、合成、包装和分泌的中心。猪的脂质代谢研究有限。本研究旨在通过转录组分析鉴定与猪脂质代谢和氧化应激相关的基因。从 60 头金华猪中采集肝段，用于测定肝脂质含量。将肝脂质含量最高和最低的 7 头猪分别设为 H 组和 L 组。从每组 H 和 L 猪中采集肝段和血清样本，用于 RNA 测序和测定甘油三酯 (TG)含量和高密度脂蛋白胆固醇 (HDL)含量。H 组猪血清中 HDL 含量明显高于 L 组 (<0.05)。从转录组测序中鉴定出 6162 个差异表达基因 (DEGs)，其中 2962 个上调，3200 个下调，随着金华猪肝含量的增加而增加。经 GO 富集和 KEGG 分析，脂质修饰、细胞脂质代谢过程、胆固醇生物合成过程、脂肪酸代谢过程、氧化还原辅酶代谢过程、氧化还原酶活性、作用于供体 CH-OH 基团、对氧化应激的反应、非酒精性脂肪肝疾病 (NAFLD)、鞘脂代谢和氧化磷酸化途径参与了金华猪的脂质代谢和氧化应激。为了进一步验证，我们选择了 10 个 DEGs 进行 RT-qPCR 验证，包括 7 个上调基因 (,,,,,, 和 ) 和 4 个下调基因 (,, 和 )。为了验证这些结果在其他猪种中的适用性，我们分析了杜洛克×长白×约克夏猪肝脏中的这 10 个 DEGs。观察到这 10 个 DEGs 的表达模式相似。这些数据将有助于深入了解调节脂质代谢和氧化应激的基因功能，并可能为通过基因调控调节脂质代谢甚至控制人类糖尿病和肥胖提供理论基础。
