Wexler I D, Hemalatha S G, Patel M S
Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
FEBS Lett. 1991 Apr 22;282(1):209-13. doi: 10.1016/0014-5793(91)80479-m.
Amino acid sequence comparison of 8 alpha and 6 beta subunits of the alpha-keto acid dehydrogenase (E1) component of the pyruvate dehydrogenase complex and branched-chain alpha-keto acid dehydrogenase complex form multiple species was performed by computer analysis. In addition to 2 previously recognized regions of homology in the alpha subunit, a 3rd region of extensive homology was identified in E1 alpha, and may be one of the sites involved in subunit interaction. E1 beta contains 4 regions of extensive homology. Region 1 contains 10 amino acids that are homologous to a 10-amino acid stretch in Escherichia coli E1. Regions 2 and 3 have sequence homologies with other dehydrogenases suggesting that these regions may be involved in catalysis.
通过计算机分析对来自多种物种的丙酮酸脱氢酶复合体和支链α-酮酸脱氢酶复合体的α-酮酸脱氢酶（E1）组分的8个α亚基和6个β亚基进行了氨基酸序列比较。除了α亚基中2个先前已识别的同源区域外，在E1α中还鉴定出了第3个广泛同源区域，该区域可能是参与亚基相互作用的位点之一。E1β包含4个广泛同源区域。区域1包含10个氨基酸，与大肠杆菌E1中的一段10个氨基酸的序列同源。区域2和区域3与其他脱氢酶具有序列同源性，表明这些区域可能参与催化作用。
