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富含鸟嘌呤-胞嘧啶基序在缺乏典型TATA盒元件的腺病毒2型IVa2启动子转录调控中的作用

Role of a GC-rich motif in transcription regulation of the adenovirus type 2 IVa2 promoter which lacks typical TATA-box element.

作者信息

Tamura T, Mikoshiba K

机构信息

Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.

出版信息

FEBS Lett. 1991 Apr 22;282(1):87-90. doi: 10.1016/0014-5793(91)80450-h.

DOI:10.1016/0014-5793(91)80450-h
PMID:2026271
Abstract

Promoter of the adenovirus type 2 IVa2 (IVa2) gene does not have a TATA-box element, hence it is considered to be a model of cellular TATA-less promoters. In vitro transcription of the IVa2 promoter in HeLa cell extracts identified a proximal cis-element around -40 relative to the transcription start site. DNase I footprint analysis revealed the presence of a DNA-binding factor which interacts with sequences ACCCCTCCCACTTAG at -50. Competition footprint analysis and sequence comparisons showed that the IVa2 proximal promoter element is equivalent to the GC-box, the Sp1-binding site.

摘要

腺病毒2型IVa2基因的启动子没有TATA盒元件，因此它被认为是细胞无TATA启动子的一个模型。在HeLa细胞提取物中对IVa2启动子进行体外转录，确定了一个相对于转录起始位点约-40处的近端顺式元件。DNase I足迹分析揭示了一种与-50处的ACCCCTCCCACTTAG序列相互作用的DNA结合因子的存在。竞争足迹分析和序列比较表明，IVa2近端启动子元件等同于GC盒，即Sp1结合位点。

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Role of a GC-rich motif in transcription regulation of the adenovirus type 2 IVa2 promoter which lacks typical TATA-box element.富含鸟嘌呤-胞嘧啶基序在缺乏典型TATA盒元件的腺病毒2型IVa2启动子转录调控中的作用
FEBS Lett. 1991 Apr 22;282(1):87-90. doi: 10.1016/0014-5793(91)80450-h.
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A TATA-like sequence located downstream of the transcription initiation site is required for expression of an RNA polymerase II transcribed gene.转录起始位点下游的类TATA序列是RNA聚合酶II转录基因表达所必需的。
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In vitro accurate initiation of transcription on the adenovirus type 2 IVa2 gene which does not contain a TATA box.在不含TATA框的腺病毒2型IVa2基因上进行体外转录的精确起始。
Nucleic Acids Res. 1982 Nov 25;10(22):7089-101. doi: 10.1093/nar/10.22.7089.

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