Vigani A, Chiara S, Miglietta L, Repetto L, Conte P F, Cimoli G, Morelli L, Billi G, Parodi S, Russo P
Department of Medical Oncology, Istituto Nazionale Ricerca sul Cancro, Genova, Italy.
Gynecol Oncol. 1991 Apr;41(1):52-5. doi: 10.1016/0090-8258(91)90254-3.
Recombinant human tumor necrosis factor (rHuTNF) is a macrophage-secretory protein with antitumor activity. In vivo and in vitro cytotoxicity studies have been carried out to test the effectiveness of rHuTNF alone or in combination with chemotherapeutic agents. We have evaluated the direct cytotoxic effect of rHuTNF on a human epithelial ovarian cancer cell line in vitro (A2774), alone, or in combination with mitoxantrone (Mit), a topoisomerase II (Topo II) targeted drug. Our results not only suggest that rHuTNF is directly cytotoxic, but also that it is able to induce a very strong potentiation of Mit cytotoxicity.
重组人肿瘤坏死因子（rHuTNF）是一种具有抗肿瘤活性的巨噬细胞分泌蛋白。已进行体内和体外细胞毒性研究，以测试rHuTNF单独或与化疗药物联合使用的有效性。我们评估了rHuTNF在体外对人上皮性卵巢癌细胞系（A2774）单独或与米托蒽醌（Mit）联合使用时的直接细胞毒性作用，米托蒽醌是一种靶向拓扑异构酶II（Topo II）的药物。我们的结果不仅表明rHuTNF具有直接细胞毒性，还表明它能够非常强烈地增强Mit的细胞毒性。
