de Vries J X, Kymber K A
Abteilung für Klinische Pharmakologie, Medizinische Klinik, Universität Heidelberg, F.R.G.
J Chromatogr. 1991 Jan 2;562(1-2):31-8. doi: 10.1016/0378-4347(91)80561-p.
Positive ion mass spectra were obtained from several coumarin oral anticoagulants (phenprocoumon, warfarin, acenocoumarol and dicoumarol) and derivatives by liquid chromatography-thermospray mass spectrometry (LC-TSP-MS) and liquid chromatography-electron impact mass spectrometry (LC-EI-MS) to assess the use of LC-MS methods for the determination of these compounds in biological materials. LC-TSP mass spectra showed a single [M + 1]+ ion with no fragmentation; LC-EI mass spectra showed fragment ions which were similar in mass and relative intensities to those obtained by conventional EI-MS. These data should serve as a basis for the development of LC-MS methods for the qualitative and quantitative analysis of coumarin anticoagulants in biological samples. LC-TSP-MS was applied to the determination of phenprocoumon in a plasma extract from an anticoagulated patient.
通过液相色谱 - 热喷雾质谱法（LC - TSP - MS）和液相色谱 - 电子轰击质谱法（LC - EI - MS）获得了几种香豆素类口服抗凝剂（苯丙香豆素、华法林、醋硝香豆素和双香豆素）及其衍生物的正离子质谱，以评估液相色谱 - 质谱法在生物材料中测定这些化合物的应用。LC - TSP质谱显示单一的[M + 1]+离子且无碎片；LC - EI质谱显示的碎片离子在质量和相对强度上与通过常规EI - MS获得的碎片离子相似。这些数据应为开发用于生物样品中香豆素类抗凝剂定性和定量分析的液相色谱 - 质谱法奠定基础。LC - TSP - MS应用于测定抗凝患者血浆提取物中的苯丙香豆素。
