Holy J M, Oaks J A, Mika-Grieve M, Grieve R
Department of Anatomy, University of Wisconsin, Madison 53706.
Parasitol Res. 1991;77(2):161-72. doi: 10.1007/BF00935431.
The epidermis of the tapeworm Hymenolepis diminuta is a highly organized syncytium, composed of an outer layer of continuous cytoplasm, or ectocytoplasm, and an inner layer of nucleated cell bodies, or perikarya. The perikarya are in direct cytoplasmic continuity with the ectocytoplasm via narrow plasmalemma-bound bridges called internuncial process. Although distinct structural and functional differences are apparent between ectocytoplasm and perikarya, all of the perikarya along the body of the cestode are morphologically similar, as are all regions of ectocytoplasm. However, immunocytochemically distinct subpopulations of perikarya and regionally defined areas of ectocytoplasm were identified along the tapeworm strobila by the use of monoclonal antibodies raised against a preparation of isolated tegument. The different types of perikarya and the regionally specialized areas of ectocytoplasm were organized in a topographically precise manner along the body of the parasite. Examination of labeling patterns after colchicine treatment suggests that different types of perikarya are specialized for biosynthesis of specific tegumental molecules and for turnover or recycling of tegumental material. Furthermore, it appears that a 52 kDa polypeptide synthesized by one population of perikarya is transported through the syncytium and ultimately resorbed by a different population of tegumental perikarya. These data suggest that the syncytial epidermis of parasitic platyhelminthes exhibits a more complex organization of function than previously appreciated.
微小膜壳绦虫的表皮是一种高度组织化的合胞体，由连续细胞质的外层（即外质）和有核细胞体的内层（即周核体）组成。周核体通过称为联络突的狭窄质膜结合桥与外质直接进行细胞质连续。尽管外质和周核体之间存在明显的结构和功能差异，但绦虫身体上所有的周核体在形态上相似，所有外质区域也是如此。然而，通过使用针对分离的皮层制备物产生的单克隆抗体，在绦虫节片中鉴定出了免疫细胞化学上不同的周核体亚群和外质的区域定义区域。不同类型的周核体和外质的区域特化区域沿着寄生虫身体以地形精确的方式组织。秋水仙碱处理后标记模式的检查表明，不同类型的周核体专门用于特定皮层分子的生物合成以及皮层物质的周转或再循环。此外，似乎由一群周核体合成的一种52 kDa多肽通过合胞体运输，最终被另一群皮层周核体吸收。这些数据表明，寄生扁形动物的合胞体表皮表现出比以前认识到的更复杂的功能组织。
