用于时间分辨荧光免疫分析的铕标记皮质醇衍生物的制备

Preparation of europium-labeled derivatives of cortisol for time-resolved fluoroimmunoassays.

作者信息

Mikola H, Miettinen P

机构信息

Wallac Chemical Laboratories, Turku, Finland.

出版信息

Steroids. 1991 Jan;56(1):17-21. doi: 10.1016/0039-128x(91)90109-9.

DOI:10.1016/0039-128x(91)90109-9

Abstract

Cortisol 3-(O-carboxymethyl)oxime, 6- and 21-hemisuccinoxycortisol, and cortisol 7-carboxyethyl thio-ether were synthesized. These carboxyl derivatives were labeled using a described general labeling method with a europium chelate. The labeled steroids were tested in a competitive time-resolved fluoroimmunoassay using antibodies raised against cortisol. Only the site-homologous antigen-antibody pairs underwent immunoreaction and gave satisfactory calibration curves.

摘要

合成了皮质醇3 -（O - 羧甲基）肟、6 - 和21 - 半琥珀酰氧基皮质醇以及皮质醇7 - 羧乙基硫醚。使用一种描述的通用标记方法用铕螯合物对这些羧基衍生物进行标记。使用针对皮质醇产生的抗体，在竞争性时间分辨荧光免疫测定中对标记的类固醇进行测试。只有位点同源的抗原 - 抗体对发生免疫反应并给出令人满意的校准曲线。

相似文献

1

Preparation of europium-labeled derivatives of cortisol for time-resolved fluoroimmunoassays.用于时间分辨荧光免疫分析的铕标记皮质醇衍生物的制备

Steroids. 1991 Jan;56(1):17-21. doi: 10.1016/0039-128x(91)90109-9.

2

Synthesis of novel europium-labeled estradiol derivatives for time-resolved fluoroimmunoassays.用于时间分辨荧光免疫分析的新型铕标记雌二醇衍生物的合成。

Bioconjug Chem. 1999 May-Jun;10(3):325-31. doi: 10.1021/bc980013q.

3

Labeling of estradiol and testosterone alkyloxime derivatives with a europium chelate for time-resolved fluoroimmunoassays.用于时间分辨荧光免疫分析的铕螯合物标记雌二醇和睾酮烷氧基肟衍生物

Steroids. 1993 Jul;58(7):330-4. doi: 10.1016/0039-128x(93)90093-3.

4

Time-resolved fluoroimmunoassay of cortisol in serum with a europium chelate as label.以铕螯合物为标记物的血清皮质醇时间分辨荧光免疫分析

Clin Biochem. 1988 Oct;21(5):291-6. doi: 10.1016/s0009-9120(88)80083-3.

5

Preparation of europium-streptavidin in a time-resolved fluoroimmunoassay for interleukin-3.用于白细胞介素-3时间分辨荧光免疫测定的铕-链霉亲和素的制备

J Immunol Methods. 1991 Apr 25;138(2):233-6. doi: 10.1016/0022-1759(91)90171-b.

6

Syntheses of europium-labeled digoxin derivatives and their use in time-resolved fluoroimmunoassay.铕标记地高辛衍生物的合成及其在时间分辨荧光免疫分析中的应用。

Steroids. 1994 Aug;59(8):472-8. doi: 10.1016/0039-128x(94)90060-4.

7

Fluorescence labelling with europium chelate of beta-diketones and application in time-resolved fluoroimmunoassays (TR-FIA).β-二酮铕螯合物荧光标记及其在时间分辨荧光免疫分析（TR-FIA）中的应用。

J Immunol Methods. 1995 Feb 27;179(2):233-41. doi: 10.1016/0022-1759(94)00289-9.

8

A novel bifunctional europium chelate applied in quantitative determination of human immunoglobin G using time-resolved fluoroimmunoassay.一种新型双功能铕螯合物在时间分辨荧光免疫分析定量测定人免疫球蛋白 G 中的应用。

Anal Biochem. 2011 Feb 15;409(2):244-8. doi: 10.1016/j.ab.2010.10.005. Epub 2010 Oct 12.

9

Direct competitive fluoroimmunoassays for detection of imidaclothiz in environmental and agricultural samples using quantum dots and europium as labels.利用量子点和铕作为标记物，直接竞争荧光免疫分析法检测环境和农业样品中的吡虫清。

Sci Total Environ. 2017 Apr 1;583:222-227. doi: 10.1016/j.scitotenv.2017.01.056. Epub 2017 Jan 20.

10

Polyvinylamine-streptavidin complexes labeled with a europium chelator: a universal detection reagent for solid-phase time resolved fluorometric applications.用铕螯合剂标记的聚乙烯胺-链霉亲和素复合物：一种用于固相时间分辨荧光分析的通用检测试剂。

Clin Biochem. 2000 Jul;33(5):345-50. doi: 10.1016/s0009-9120(00)00082-5.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。