Ci Y X, Yang X D, Chang W B
Department of Chemistry, Peking University, Beijing, People's Republic of China.
J Immunol Methods. 1995 Feb 27;179(2):233-41. doi: 10.1016/0022-1759(94)00289-9.
Five beta-diketone derivatives were studied for multiple labelling of proteins. The labelled proteins were characterized by absorption and fluorescence measurements. It was found that proteins labelled with chlorosulfonyl-thenoyltrifluoroacetone (CTTA) were able to form highly fluorescent complexes with Eu3+ which exhibited prolonged fluorescence whereas the Eu3+ complex of hydrolyzed CTTA exhibited almost no fluorescence, and so unreacted ligand gave no background signal in immunoassays even if it was not removed from the labelled reagent. The effect of labelling on the biological activity of albumin and polyclonal antibody was studied and it was also shown that the new probe could be used in time-resolved fluorescence immunoassays.
研究了五种β-二酮衍生物用于蛋白质的多重标记。通过吸收和荧光测量对标记的蛋白质进行了表征。发现用氯磺酰基 - 噻吩甲酰三氟丙酮（CTTA）标记的蛋白质能够与Eu3+形成高荧光复合物，该复合物表现出延长的荧光，而水解的CTTA的Eu3+复合物几乎没有荧光，因此即使未从标记试剂中去除未反应的配体，其在免疫测定中也不会产生背景信号。研究了标记对白蛋白和多克隆抗体生物活性的影响，还表明新探针可用于时间分辨荧光免疫测定。
