Wang L F, Lu Y Q, Fu M Z
Department of Biochemistry, Hunan Medical University, Changsha.
Yi Chuan Xue Bao. 1991;18(1):1-5.
A screening survey of variant glycophorins (GP) on the erythrocyte membranes was conducted among 114 healthy Chinese young people who belong to several different ethnic groups by immunoblotting techniques. The majority (70) were of the Han ethnic group; 24 out of 41 Hainanese belonged to the Li ethnic group. Ten subjects showed variant GP profiles, with an incidence of 8.8%. The frequency of variants was significantly higher in the Li ethnic group (25%) than in the Han ethnic group (5.7%); none was seen among the other ethnic minorities. Probing with four antibodies specific to different regions of GP molecules revealed three distinct types of GP among the variant individuals. Type I observed in four individuals was reminiscent of that exhibited by Sta glycophorin. Among them, in one subject the Sta blood group phenotype was confirmed by serological typing. The absence of delta GP was noted in another subject carrying type II variant GP. Patterns in the remaining five individuals (type III) could not be correlated with known GP profiles of variants of MNSs blood system. This study suggests that there may be a high incidence of GP variants among certain ethnic groups in China. The sialic acid contents of erythrocyte membranes in seven GP propositi were significantly higher than those of the control.
采用免疫印迹技术，对114名来自几个不同民族的健康中国年轻人进行了红细胞膜上变异血型糖蛋白（GP）的筛查调查。其中大多数（70人）为汉族；41名海南人中24人属于黎族。10名受试者呈现变异GP谱，发生率为8.8%。黎族变异频率（25%）显著高于汉族（5.7%）；其他少数民族未发现变异。用四种针对GP分子不同区域的特异性抗体进行检测，在变异个体中发现了三种不同类型的GP。在四名个体中观察到的I型类似于Sta血型糖蛋白所呈现的类型。其中，一名受试者通过血清学分型证实为Sta血型。在另一名携带II型变异GP的受试者中，发现缺乏δGP。其余五名个体（III型）的模式与MNSs血型系统变异的已知GP谱不相关。本研究表明，中国某些民族中GP变异的发生率可能较高。7名GP先证者红细胞膜的唾液酸含量显著高于对照组。
