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[由整合在预定位点的微小F质粒引发的大肠杆菌染色体复制对recA基因的依赖性]

[recA gene dependence of chromosome replication of Escherichia coli initiated by mini-F plasmids integrated at predetermined sites].

作者信息

Mao Y M, Sheng Z J

机构信息

Institute of Genetics, Fudan University, Shanghai.

出版信息

Yi Chuan Xue Bao. 1991;18(1):73-81.

PMID:2029428
Abstract

mini-F plasmids carrying particular chromosomal fragments were constructed. Through homologous recombination they were expected to integrate onto predetermined sites of the chromosome of dnaA46 strain LC381. Chromosome replication of suppressive integration (Sin) strains were recA dependent or independent according to the site of integration of the plasmid: chromosome replication of those with the plasmid integrated close to oriC were found to be recA dependent at 40 degrees C; those with the plasmid integrated between oriC and terC were recA dependent on rich medium but independent on minimal medium; those with the plasmid integrated close to terC were recA dependent even on minimal medium.

摘要

构建了携带特定染色体片段的微型F质粒。通过同源重组，预计它们会整合到dnaA46菌株LC381染色体的预定位点上。抑制性整合（Sin）菌株的染色体复制根据质粒的整合位点是recA依赖性或非依赖性的：发现质粒整合在oriC附近的菌株在40℃时染色体复制是recA依赖性的；质粒整合在oriC和terC之间的菌株在丰富培养基上是recA依赖性的，但在基本培养基上是非依赖性的；质粒整合在terC附近的菌株即使在基本培养基上也是recA依赖性的。

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1
[recA gene dependence of chromosome replication of Escherichia coli initiated by mini-F plasmids integrated at predetermined sites].[由整合在预定位点的微小F质粒引发的大肠杆菌染色体复制对recA基因的依赖性]
Yi Chuan Xue Bao. 1991;18(1):73-81.
2
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[Through homologous recombination pathway recA gene takes part in chromosome replication of E. coli initiated by integrated plasmid].通过同源重组途径，recA基因参与由整合质粒启动的大肠杆菌染色体复制。
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