灵长类D型逆转录病毒主要结构蛋白的种间放射免疫测定
Interspecies radioimmunoassay for the major structural proteins of primate type-D retroviruses.
作者信息
Colcher D, Teramoto Y A, Schlom J
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5739-43. doi: 10.1073/pnas.74.12.5739.
A competition radioimmunoassay has been developed in which type-D retroviruses from three primate species compete. The assay utilizes the major structural protein (36,000 daltons) of the endogenous squirrel monkey retrovirus and antisera directed against the major structural protein (27,000 daltons) of the Mason-Pfizer monkey virus isolated from rhesus monkeys. Purified preparations of both viruses grown in heterologous cells, as well as extracts of heterologous cells infected with squirrel monkey retrovirus or Mason-Pfizer monkey virus, compete completely in the assay. Addition of an endogenous virus of the langur monkey also results in complete blocking. No blocking in the assay is observed with type-C baboon viruses, woolly monkey virus, and gibbon virus. Various other type-C and type-B viruses also showed no reactivity. An interspecies assay has thus been developed that recognizes the type-D retroviruses from both Old World monkey (rhesus and langur) and New World monkey (squirrel) species.
已开发出一种竞争性放射免疫测定法，其中来自三种灵长类物种的D型逆转录病毒相互竞争。该测定法利用内源性松鼠猴逆转录病毒的主要结构蛋白（36,000道尔顿）以及针对从恒河猴分离出的梅森 - 辉瑞猴病毒的主要结构蛋白（27,000道尔顿）的抗血清。在异源细胞中生长的两种病毒的纯化制剂，以及感染了松鼠猴逆转录病毒或梅森 - 辉瑞猴病毒的异源细胞提取物，在测定中完全竞争。添加叶猴的内源性病毒也会导致完全阻断。在测定中未观察到C型狒狒病毒、绒毛猴病毒和长臂猿病毒的阻断作用。各种其他C型和B型病毒也没有反应性。因此，已开发出一种种间测定法，可识别来自旧世界猴（恒河猴和叶猴）和新世界猴（松鼠猴）物种的D型逆转录病毒。
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