Stellar J R, Hall F S, Waraczynski M
Department of Psychology, Northeastern University, Boston, MA 02215.
Brain Res. 1991 Feb 8;541(1):29-40. doi: 10.1016/0006-8993(91)91070-h.
Unilateral microinjection into rat lateral hypothalamus (LH) of the excitotoxins ibotenic acid (IBO) and N-methyl-D-aspartic acid (NMDA) produced a local zone of neuronal death but also produced a zone of demyelination. The size of this demyelination zone was related to excitotoxin dose and was smaller than the zone of neuron killing. In behavioral testing, MFB self-stimulation reward and performance were measured with a rate-frequency curve-shift method before and after IBO or NMDA lesions of the LH. Excitotoxin lesions were made anterior or posterior to the LH electrode so that the zone of neuronal death, but not demyelination, extended to the electrode tip. These lesions produced small, temporary LH stimulation reward deficits, leading to the conclusion that intrinsic LH neurons are not a major substrate of MFB stimulation reward.
向大鼠外侧下丘脑（LH）单侧微量注射兴奋性毒素鹅膏蕈氨酸（IBO）和N-甲基-D-天冬氨酸（NMDA），不仅造成了局部神经元死亡区域，还产生了脱髓鞘区域。该脱髓鞘区域的大小与兴奋性毒素剂量相关，且小于神经元杀伤区域。在行为测试中，采用速率-频率曲线偏移法，在LH接受IBO或NMDA损伤前后，测量内侧前脑束（MFB）自我刺激奖赏和行为表现。兴奋性毒素损伤位于LH电极的前方或后方，使得神经元死亡区域（而非脱髓鞘区域）延伸至电极尖端。这些损伤导致了轻微、短暂的LH刺激奖赏缺陷，从而得出结论：LH内在神经元并非MFB刺激奖赏的主要底物。
