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土耳其北部野生鸟类中禽流感病毒的分子检测,但未检测到西尼罗河病毒。

Molecular detection of avian influenza virus but not West Nile virus in wild birds in northern Turkey.

机构信息

Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey.

出版信息

Zoonoses Public Health. 2010 Dec;57(7-8):e71-5. doi: 10.1111/j.1863-2378.2010.01327.x.

Abstract

In this study, a total of 402 samples (173 cloacal swab samples, 169 tracheal swab samples and 60 organ pools including the lung, spleen, liver, trachea and brain) obtained from 27 different wild avian species from Kizilirmak delta and the adjacent wetlands in northern Turkey were surveyed for the presence of RNA from Avian influenza virus (AIV) and West Nile virus (WNV) by Taqman-based real-time reverse transcriptase-polymerase chain reaction assay. No WNV genomic RNA was detected in any sample. In contrast, AIV RNA was found in two of 402 samples (0.49%).

摘要

在这项研究中,对来自土耳其北部基济尔拉克三角洲和邻近湿地的 27 种不同野生鸟类的 402 个样本(173 个泄殖腔拭子样本、169 个气管拭子样本和 60 个器官池,包括肺、脾、肝、气管和脑)进行了检测,以确定是否存在禽流感病毒(AIV)和西尼罗河病毒(WNV)的 RNA,方法是基于 Taqman 的实时逆转录聚合酶链反应检测。在任何样本中均未检测到 WNV 基因组 RNA。相比之下,在 402 个样本中的两个样本中发现了 AIV RNA(0.49%)。

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