Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi, Nagasaki, Japan.
Talanta. 2010 May 15;81(3):1133-6. doi: 10.1016/j.talanta.2010.01.055. Epub 2010 Feb 1.
Enhancement of peroxyoxalate chemiluminescence (PO-CL) intensity by a surfactant in the H(2)O(2)/bis(2,4,6-trichlorophenyl)oxalate (TCPO)/rhodamine B system was described. The effects of 15 surfactants were evaluated by comparing the ratio of a relative CL intensity (RCI) with surfactant to that of the blank in each system. In preliminary study, H(2)O(2)/imidazole-HNO(3) buffer/TCPO/rhodamine B system was used to study the effects of surfactants on PO-CL intensity. Fourteen surfactants reduced the CL intensity at the 2% concentration, where their relative CL intensities ranged from 0.6 to 93.5%. Some of these phenomena may be caused by a notable change of pH that was occurred by adding the surfactant. Additionally, enhancement of PO-CL intensity was studied by using system (1) H(2)O(2)/TCPO/rhodamine B and (2) H(2)O(2)/imidazole-HNO(3) buffer/TCPO/systems. In the system 1, the favorable enhancement of CL intensity (ranged from 124 to 472%) was observed with 9 surfactants at the 0.5% concentration. This result suggested that several surfactants might play a role as a catalyst in the PO-CL reaction. There was no tendency to enhance CL intensity among the surfactant types. In the system 2, the enhancement of CL intensity was also observed by adding with 11 surfactants, which might be mainly caused by the fluorescent impurities of surfactants used. Furthermore, detection of detergent commercially available was applied by using the system 1.
该文描述了在 H(2)O(2)/双(2,4,6-三氯苯基)草酸酯(TCPO)/罗丹明 B 体系中，表面活性剂增强过氧草酸酯化学发光（PO-CL）强度的作用。通过比较每个体系中含表面活性剂的相对 CL 强度（RCI）与空白的比值，评估了 15 种表面活性剂的影响。在初步研究中，使用 H(2)O(2)/咪唑-HNO(3)缓冲液/TCPO/罗丹明 B 体系研究了表面活性剂对 PO-CL 强度的影响。14 种表面活性剂在 2%浓度下降低了 CL 强度，其相对 CL 强度范围为 0.6 至 93.5%。这些现象中的一些可能是由于添加表面活性剂引起的 pH 值发生明显变化所致。此外，还通过使用体系（1）H(2)O(2)/TCPO/罗丹明 B 和（2）H(2)O(2)/咪唑-HNO(3)缓冲液/TCPO/体系研究了 PO-CL 强度的增强。在体系 1 中，在 0.5%浓度下，有 9 种表面活性剂观察到 CL 强度的有利增强（范围为 124%至 472%）。这一结果表明，几种表面活性剂可能在 PO-CL 反应中起到催化剂的作用。不同类型的表面活性剂之间没有增强 CL 强度的趋势。在体系 2 中，通过添加 11 种表面活性剂也观察到 CL 强度的增强，这可能主要是由于所用表面活性剂的荧光杂质所致。此外，还应用该体系 1 检测了市售洗涤剂。
