逆转录聚合酶链反应（RT-PCR）诊断后对蛇类临床样本中的副黏病毒进行序列特征分析，揭示了群体内和/或个体内的同时感染。

RT-PCR diagnosis followed by sequence characterization of paramyxoviruses in clinical samples from snakes reveals concurrent infections within populations and/or individuals.

机构信息

Institut für Umwelt- und Tierhygiene, University of Hohenheim, Stuttgart, Germany.

出版信息

Vet Microbiol. 2010 Aug 26;144(3-4):466-72. doi: 10.1016/j.vetmic.2010.02.002. Epub 2010 Feb 6.

DOI:10.1016/j.vetmic.2010.02.002

PMID:20299166

Abstract

Paramyxoviruses (PMV) are important causes of disease in snakes in both private and zoologic collections and have been described in many different parts of the world. We have used the described L-gene nested RT-PCR (Ahne et al., 1999) as a diagnostic tool for the detection and characterization of PMV in clinical field samples from 102 snakes from Germany and Hungary. Fifty of the tested 203 samples were positive in the RT-PCR. 38 organs or swab samples from 25 snakes were selected for sequencing. They resulted in 13 different partial L-gene sequences, eight of which were identical to earlier characterized isolates, while five were new unique sequences, but also closely related to the earlier described PMV sequences. The comparison of sequences obtained from tissues from the same animal or from different animals from the same collection indicates that snakes can be infected with several different PMV during one outbreak. This is the first report of concurrent infection in a single snake or group of snakes with several distinct PMV.

摘要

副黏病毒（PMV）是私人和动物园收藏中蛇类疾病的重要原因，在世界许多不同地区都有描述。我们使用描述的 L 基因巢式 RT-PCR（Ahne 等人，1999 年）作为检测和鉴定临床现场样本中 PMV 的诊断工具，这些样本来自德国和匈牙利的 102 条蛇。在测试的 203 个样本中，有 50 个在 RT-PCR 中呈阳性。从 25 条蛇的 38 个器官或拭子样本中选择进行测序。结果产生了 13 个不同的部分 L 基因序列，其中 8 个与之前表征的分离物相同，而 5 个是新的独特序列，但也与之前描述的 PMV 序列密切相关。来自同一动物的组织或来自同一收藏中的不同动物的序列比较表明，蛇在一次暴发中可能感染几种不同的 PMV。这是首次在单个蛇或一组蛇中同时感染几种不同的 PMV 的报告。