Jerome W G, Minor L K, Glick J M, Rothblat G H, Lewis J C
Department of Pathology, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103.
Exp Mol Pathol. 1991 Apr;54(2):144-58. doi: 10.1016/0014-4800(91)90027-u.
Using an inverted culture technique, the accumulation of lipid within vascular smooth muscle cells incubated with lipid droplets was studied. Initially, lipid was found exclusively within cytoplasmic inclusions but, as accumulation continued, lysosomes became the predominant site of lipid storage. After 3 hr of incubation, 84% of lipid was within lysosomes. This lysosomal lipid accumulation produced a tripling of the average size of lysosomes and resulted in lysosomes with complex, multilobed shapes. In contrast, although the number of cytoplasmic inclusions increased with lipid loading, individual inclusions maintained a spherical shape and a consistent diameter of 1-1.3 microns. Concomitant with changes in cellular lipid storage, incubation with lipid droplets induced development of an anastomosing network of acid phosphatase-containing tubules which were spatially related to sites of lysosomal lipid accumulation. Thus lipid accumulation produced ultrastructural alterations in a number of metabolic compartments. Similar alterations in the intracellular compartmentalization of acquired lipid have been demonstrated in foam cells during atherogenesis and have been hypothesized to have profound effects on lipid metabolism and disease progression.
采用倒置培养技术，研究了与脂滴一起孵育的血管平滑肌细胞内脂质的积累情况。最初，脂质仅存在于细胞质内含物中，但随着积累的持续，溶酶体成为脂质储存的主要部位。孵育3小时后，84%的脂质位于溶酶体内。这种溶酶体脂质积累使溶酶体的平均大小增加了两倍，并导致溶酶体呈现复杂的多叶形状。相比之下，尽管细胞质内含物的数量随着脂质负载而增加，但单个内含物保持球形，直径一致为1-1.3微米。与细胞脂质储存的变化相伴，与脂滴孵育诱导了含酸性磷酸酶的小管吻合网络的形成，这些小管在空间上与溶酶体脂质积累部位相关。因此，脂质积累在许多代谢区室中产生了超微结构改变。在动脉粥样硬化形成过程中，泡沫细胞中也已证明获得性脂质的细胞内区室化存在类似改变，并据推测这对脂质代谢和疾病进展具有深远影响。
