Nagao Tamiko, Yamashita Tomoki, Miyake Ariko, Uchiyama Tsuneo, Nomaguchi Masako, Adachi Akio
Department of Microbiology, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
J Med Invest. 2010 Feb;57(1-2):89-94. doi: 10.2152/jmi.57.89.
We examined a series of site-directed point mutants of human immunodeficiency virus type 1 (HIV-1) Vif for their interaction with cellular anti-viral factors APOBEC3G/APOBEC3F. Mutant viruses that display growth-defect in H9 cells did not counteract effectively APOBEC3G and/or APOBEC3F without exception, as monitored by single-cycle infectivity assays. While growth-defective mutants of Vif C-terminal region were unable to suppress APOBEC3G/APOBEC3F, some N-terminal region mutants did neutralize one of APOBEC3G/APOBEC3F. These data have suggested that members of APOBEC3 family other than APOBEC3G/APOBEC3F are not important for anti-HIV-1 activity. Furthermore, APOPEC3G/APOBEC3F were found to differently associate with Vif in virions as analyzed by equilibrium density centrifugation. Taken together, these results indicated that interaction of HIV-1 Vif and APOBEC3G is distinct from that between Vif and APOBEC3F.
我们检测了一系列人免疫缺陷病毒1型（HIV-1）Vif的定点突变体与细胞抗病毒因子载脂蛋白B mRNA编辑酶催化多肽样3G/载脂蛋白B mRNA编辑酶催化多肽样3F（APOBEC3G/APOBEC3F）的相互作用。通过单循环感染性测定监测发现，在H9细胞中表现出生长缺陷的突变病毒无一例外地不能有效对抗APOBEC3G和/或APOBEC3F。虽然Vif C末端区域的生长缺陷突变体无法抑制APOBEC3G/APOBEC3F，但一些N末端区域突变体确实能中和APOBEC3G/APOBEC3F中的一种。这些数据表明，除APOBEC3G/APOBEC3F外的APOBEC3家族成员对抗HIV-1活性并不重要。此外，通过平衡密度离心分析发现，APOPEC3G/APOBEC3F在病毒颗粒中与Vif的结合方式不同。综上所述，这些结果表明HIV-1 Vif与APOBEC3G的相互作用不同于Vif与APOBEC3F之间的相互作用。
