Sakaki Manabu, Fukumori Tomoharu, Fukawa Tomoya, Elsamman Essam, Shiirevnyamba Avirmed, Nakatsuji Hiroyoshi, Kanayama Hiro-omi
Department of Urology, Institute of Health Biosciences, University of Tokushima Graduated School, Tokushima, Japan.
J Med Invest. 2010 Feb;57(1-2):152-7. doi: 10.2152/jmi.57.152.
Overexpression of galectin-3 in a variety of cancer cell lines has been shown to correlate with tumor progression and metastasis. In this study, we investigated the expression of galectin-3 in clear cell renal cell carcinoma (CC-RCC) and evaluated the relationship between galectin-3 expression levels and the clinicopathological features of CC-RCC. Expression of galectin-3 in the kidney cancer cell lines Caki-1, Caki-2, A704, ACHN and KPK-1 were evaluated using western blot analysis, while galectin-3 expression in CC-RCC tissues and normal parenchyma were measured by real-time PCR and immunohistochemistry. We found that galectin-3 was overexpressed in the Caki-1, Caki-2, A704, ACHN and KPK-1 cell lines and that the expression level in CC-RCC was also significantly higher than that in renal parenchyma obtained from the same patient samples (p=0.039). Galectin-3 expression in CC-RCC with distant metastasis was also significantly higher than that in CC-RCC without distant metastasis (p=0.045). In conclusion, we revealed that galectin-3 is highly expressed in CC-RCC, especially in CC-RCC with distant metastasis, suggesting that galectin-3 may serve as a novel target molecule for predicting CC-RCC metastasis.
在多种癌细胞系中，半乳糖凝集素-3的过表达已被证明与肿瘤进展和转移相关。在本研究中，我们调查了半乳糖凝集素-3在透明细胞肾细胞癌（CC-RCC）中的表达，并评估了半乳糖凝集素-3表达水平与CC-RCC临床病理特征之间的关系。采用蛋白质印迹分析评估半乳糖凝集素-3在肾癌细胞系Caki-1、Caki-2、A704、ACHN和KPK-1中的表达，同时通过实时PCR和免疫组织化学检测CC-RCC组织和正常实质中半乳糖凝集素-3的表达。我们发现半乳糖凝集素-3在Caki-1、Caki-2、A704、ACHN和KPK-1细胞系中过表达，并且CC-RCC中的表达水平也显著高于来自相同患者样本的肾实质中的表达水平（p = 0.039）。伴有远处转移的CC-RCC中半乳糖凝集素-3的表达也显著高于无远处转移的CC-RCC（p = 0.045）。总之，我们揭示了半乳糖凝集素-3在CC-RCC中高表达，尤其是在伴有远处转移的CC-RCC中，这表明半乳糖凝集素-3可能作为预测CC-RCC转移的新型靶分子。
