Department of Electrical and Computer Engineering, University of Alberta, Edmonton T6G 2V4, Canada.
Cytometry A. 2010 Jun;77(6):580-4. doi: 10.1002/cyto.a.20891.
A finite-difference time-domain (FDTD) method is used to study the multiple scattering from many organelle-size particles distributed in a biological cell. Conventional flow cytometry, where the small-angle forward scatter (FSC) intensity and side scatter (SSC) intensity are used for cell characterizations, may have difficulties to differentiate the organelle distributions in biological cells. Based on the FDTD simulations, a light-scattering methodology is proposed here to overcome such a problem. This method differentiates the dense and sparse distributions of organelle-size particles in a cell, by counting the peak numbers in both large-angle FSC and wide-angle SSC, with the multiple scattering effects being considered. Implemented with a wide-angle microfluidic cytometer, the approach demonstrated in this theoretical study may find potential applications in clinics for label-free cell physiological study.
采用时域有限差分（FDTD）方法研究了分布在生物细胞中的多个细胞器大小颗粒的多次散射。传统的流式细胞术使用小角度前向散射（FSC）强度和侧向散射（SSC）强度来进行细胞特征分析，可能难以区分生物细胞中的细胞器分布。基于 FDTD 模拟，本文提出了一种光散射方法来克服这个问题。该方法通过在大角度 FSC 和广角 SSC 中计算峰值数量，同时考虑多次散射效应，区分细胞中细胞器大小颗粒的密集和稀疏分布。在广角微流控细胞仪上实现，本理论研究中提出的方法可能在临床中用于无标记细胞生理研究中具有潜在的应用价值。
