[The effect of cigarette smoking on serum concentration of conjugated dienes in stroke patients].

Exposition to tobacco smoke as a source of free radicals leads to stimulation of lipids peroxidation. Oxidation of low-density lipoproteins (LDL) increases the risk of stroke. Aim of the study was to evaluate the effect of cigarette smoking on the serum concentration of conjugated dienes as markers of peroxidation of fatty acids. In the study participated 431 subsequent stroke patients (ischemic and with hemorrhagic transformation) hospitalized in Department of Neurology in Poznan in a period from February 2007 to may 2008. The control group consisted of 16 healthy, non-smoking volunteers. The concentration of conjugated dienes (CD) in patients' sera was estimated by spectrophotometric methods. Furthermore, lipid profile, glucose level, concentration of C-reactive protein and white blood cells count were analysed. We have also calculated waist- to hip ratio. Serum concentrations of conjugated dienes were increased both in smoking and non-smoking patients with ischemic stroke. We noticed negative correlation between conjugated dienes levels and tobacco smoking duration in patients with ischemic stroke (Kendall's tau = -0.154, p = 0.0474). Moreover, conjugated dienes concentration correlated positively with triacylglycerides levels in smokers with ischemic stroke (rSpearman = 0.198, p = 0.0452; Kendall's tau = 0.135, p = 0.0428). The abnormalities in concentrations of conjugated dienes in ischemic stroke patients resulted rather from disturbances of cerebral circulation, than from cigarette smoking itself. Hypertriglyceridemia in smokers with ischemic stroke causes up-regulation of lipids peroxidation. The rate of production of conjugated dienes is associated with distribution of adipose tissue reflected by waist to hip ratio.