Piekoszewski Wojciech, Florek Ewa, Kobus Andrzej, Lechowicz Wojciech, Blanquart Timothee
Department of Analytical Chemistry, Jagiellonian University, Krakow, Poland.
Przegl Lek. 2009;66(10):850-2.
Opium derivatives as the addictive substances cause many medical and social problems. In treatment of opiates addiction except psychotherapy a long time pharmacotherapy with methadone or buprenorphine are apply. In a recent study both moderate- and high-dose methadone treatment resulted in reductions in illicit opioid use. The aim of the study was develop fast high sensitive liquid chromatography coupled with mass spectrometry for determination of methadone and EDDP after microwave assisted extraction. Developed method for methadone and EDDP determination were linear over the range 5 to 1000 ng/ml for methadone and its metabolite. The method exhibit a good intra and inter day precision, the microwave assisted extraction was also satisfactory with recovery superior to 80%. The mean concentration of methadone in plasma of patients receiving 40 mg of drug per day was 194 ng/ml but in second group (receiving 90 mg of methadone/day) 263 ng/ml. The ratio of the doses was 2.25 but for methadone concentration only 1.36. The much higher ratio (3.25) of concentrations was noticed for EDDP. This observation can support opinion other authoress about polymorphism of methadone pharmacokinetics and pharmacodynamics.
鸦片衍生物作为成瘾性物质会引发诸多医学和社会问题。在阿片类药物成瘾的治疗中，除心理治疗外，还会长期应用美沙酮或丁丙诺啡进行药物治疗。在最近的一项研究中，中等剂量和高剂量美沙酮治疗均导致非法阿片类药物使用量减少。该研究的目的是开发一种快速、高灵敏度的液相色谱-质谱联用方法，用于在微波辅助萃取后测定美沙酮和乙二胺二苯酮（EDDP）。所开发的美沙酮和EDDP测定方法在美沙酮及其代谢物浓度范围为5至1000 ng/ml时呈线性。该方法具有良好的日内和日间精密度，微波辅助萃取效果也令人满意，回收率超过80%。每天接受40 mg药物治疗的患者血浆中美沙酮的平均浓度为194 ng/ml，但在第二组（每天接受90 mg美沙酮治疗）中为263 ng/ml。剂量比为2.25，但美沙酮浓度比仅为1.36。对于EDDP，观察到浓度比更高（3.25）。这一观察结果可以支持其他作者关于美沙酮药代动力学和药效学多态性的观点。
