Ping Wang, Liu Xiang-nanz, Huang Fang
School of Urban and Environmental Sciences, Northeast Normal University, Changchun, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Jan;30(1):197-201.
Chlorophyll content is an important indicator of photosynthesis activity, stress and nutritional state. In the present paper, the hyperspectral data, foliar chlorophyll content and heavy metal contents in foliar and soil were measured for the maize growing in three natural fields. In most previous research, the contamination stress was controlled artificially in laboratory by adding chromium, zinc or copper pollutant etc. to the soil, and the pollutant concentration added was much higher than that in natural environment. The three sample fields were under different heavy mental contamination level, but all located at the Changchun region, Northeast China, where is called Golden Maize Belts in the world. After continuum removal (400-800 nm), ten spectral indices were computed including max absorption position, normalized reflectance at max absorption position, absorption depth, green peak, normalized reflectance at green peak, red edge, normalized reflectance at red edge, red peak, absorption width, and asymmetry degree. The physics meaning of the above indices and their correlation with maize foliar chlorophyll content were analyzed. It was found that there were close relationships between these indices and foliar chlorophyll content except max absorption position, green edge and asymmetry degree. Besides the asymmetry degree, five indices were selected in the stepwise multiple linear regression for estimating chlorophyll content and its determination coefficient (R2) is 0.7027. Furthermore, in order to measure the weak change information of foliar chlorophyll content under the contamination stress, the BP artificial neural network (ANN-BP) was used. Several ANN-BP models were built and tried with different structure, namely five nodes, seven nodes or ten nodes in input layer, one hidden layer or two hidden layer, and different nodes number in hidden layers. It was found that the highest accuracy of estimates was obtained by the model with two hidden layers, ten nodes in input layer, seven nodes in first hidden layer and 4 nodes in second hidden layer (R2 = 0.9758).
叶绿素含量是光合作用活性、胁迫和营养状况的重要指标。在本文中,对三块天然田地里生长的玉米进行了高光谱数据、叶片叶绿素含量以及叶片和土壤中重金属含量的测定。在之前的大多数研究中,污染胁迫是在实验室中通过向土壤中添加铬、锌或铜污染物等进行人工控制的,添加的污染物浓度远高于自然环境中的浓度。这三块样地处于不同的重金属污染水平,但都位于中国东北的长春地区,该地区被称为世界黄金玉米带。经过连续去除(400 - 800纳米)后,计算了十个光谱指数,包括最大吸收位置、最大吸收位置处的归一化反射率、吸收深度、绿峰、绿峰处的归一化反射率、红边、红边处的归一化反射率、红峰、吸收宽度和不对称度。分析了上述指数的物理意义及其与玉米叶片叶绿素含量的相关性。结果发现,除最大吸收位置、绿边和不对称度外,这些指数与叶片叶绿素含量之间存在密切关系。除不对称度外,在逐步多元线性回归中选择了五个指数来估算叶绿素含量,其决定系数(R2)为0.7027。此外,为了测量污染胁迫下叶片叶绿素含量的微弱变化信息,使用了BP人工神经网络(ANN - BP)。构建并尝试了几个具有不同结构的ANN - BP模型,即输入层有五个节点、七个节点或十个节点,一个隐藏层或两个隐藏层,以及隐藏层中不同的节点数。结果发现,具有两个隐藏层、输入层十个节点、第一个隐藏层七个节点和第二个隐藏层四个节点的模型获得了最高的估计精度(R2 = 0.9758)。