von Einem S, Schwarz E, Rudolph R
Institute for Biochemistry and Biotechnology, Kurt-Mothes-Str. 3, Martin-Luther University Halle-Wittenberg, 06120 Halle, Germany.
Protein Expr Purif. 2010 Sep;73(1):65-9. doi: 10.1016/j.pep.2010.03.009. Epub 2010 Mar 17.
Bone morphogenetic proteins (BMPs) stimulate bone formation and thus constitute important protein therapeutics. Here, a novel procedure is presented which allows fast and efficient production of biologically active BMP-2 via a TWO-STEP procedure: the conditions are designed such that the first step favors formation of monomeric species with the correct intramolecular disulfide bridges, the conditions of the second folding reaction stimulate the formation of the intermolecular disulfide bridge. The short processing times and increased yields compared to previously published procedures allow low-cost production of this important protein drug.
骨形态发生蛋白（BMPs）可刺激骨形成，因此是重要的蛋白质治疗药物。在此，我们提出了一种新方法，该方法通过两步法能够快速高效地生产具有生物活性的BMP-2：设计反应条件，使第一步有利于形成具有正确分子内二硫键的单体物种，第二步折叠反应的条件则刺激分子间二硫键的形成。与先前发表的方法相比，该方法处理时间短且产率提高，能够低成本生产这种重要的蛋白质药物。
